Nitrophenols suppress steroidogenesis in prehierarchical chicken ovarian follicles by targeting STAR, HSD3B1, and CYP19A1 and downregulating LH and estrogen receptor expression

Andrzej Sechman , Agnieszka Grzegorzewska , Małgorzata Grzesiak , Anna Kozubek , Dorota Katarzyńska-Banasik , Kinga Kowalik , Anna Hrabia

Abstract

To assess the effects of 4-nitrophenol (PNP) and 3-methyl-4-nitrophenol (PNMC) on steroidogenesis in the chicken ovary, white (WF, 1–4 mm) and yellowish (YF, 4–8 mm) prehierarchical follicles were incubated in a medium supplemented with PNP or PNMC (10−8–10−4 M), ovine LH (oLH; 10 ng/mL), and combinations of oLH with PNP or PNMC (10−6 M). Testosterone (T) and estradiol (E2) concentrations in media and mRNA expression for steroidogenic proteins (STAR, HSD3B1, and CYP19A1), and LH receptors (LHR), estrogen receptor α (ESR1) and β (ESR2) in follicles were determined by RIA and real-time qPCR, respectively. PNP and PNMC decreased T and E2 secretion by the WF and YF, and oLH-stimulated T secretion from these follicles. PNP decreased basal STAR and HSD3B1 mRNA levels both in the WF and YF, and CYP19A1 mRNAs in the WF. PNP reduced oLH-affected mRNA expression of these genes in the YF. PNMC inhibited basal STAR, HSD3B1, and CYP19A1 mRNA expression in the WF, but not in the YF. PNMC reduced oLH-stimulated STAR and CYP19A1 expression in the YF and WF, respectively. PNP decreased basal mRNA expression of LHR, ESR1, and ESR2 in the WF, but it increased ESR1 and ESR2 mRNA levels in the YF. PNMC reduced both basal and oLH-affected LHR, ESR1, and ESR2 mRNA expression in the WF; however, it did not influence expression of these genes in the YF. We suggest that nitrophenols by influencing sex steroid synthesis and transcription of LH and estrogen receptors in prehierarchical ovarian follicles may impair their development and selection to the preovulatory hierarchy.
Author Andrzej Sechman (FoAS / DoAPaE)
Andrzej Sechman,,
- Department of Animal Physiology and Endocrinology
, Agnieszka Grzegorzewska (DoAS / DoAPaE)
Agnieszka Grzegorzewska,,
- Department of Animal Physiology and Endocrinology
, Małgorzata Grzesiak (FoAS / DoAPaE)
Małgorzata Grzesiak,,
- Department of Animal Physiology and Endocrinology
, Anna Kozubek (FoAS / DoAPaE)
Anna Kozubek,,
- Department of Animal Physiology and Endocrinology
, Dorota Katarzyńska-Banasik (FoAS / DoAPaE)
Dorota Katarzyńska-Banasik,,
- Department of Animal Physiology and Endocrinology
, Kinga Kowalik (DoAS / DoAPaE)
Kinga Kowalik,,
- Department of Animal Physiology and Endocrinology
, Anna Hrabia (FoAS / DoAPaE)
Anna Hrabia,,
- Department of Animal Physiology and Endocrinology
Journal seriesDomestic Animal Endocrinology, ISSN 0739-7240, e-ISSN 1879-0054, (N/A 70 pkt)
Issue year2020
Vol70
Pages1-11
Publication size in sheets0.5
Article number106378
Keywords in EnglishNitrophenols, Steroidogenesis, LHR and ER receptors, Chicken ovary
ASJC Classification3403 Food Animals; 1103 Animal Science and Zoology; 1310 Endocrinology
DOIDOI:10.1016/j.domaniend.2019.07.006
URL https://www.sciencedirect.com/science/article/pii/S0739724019300499/pdfft?md5=9d7d5d139b9790a8d018d4dc79419cd7&pid=1-s2.0-S0739724019300499-main.pdf
Languageen angielski
Score (nominal)70
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.955; WoS Impact Factor: 2018 = 2.302 (2) - 2018=2.063 (5)
Citation count*
Additional fields
FinansowanieThis work was financially supported by the National Science Center, Poland (grant no. UMO-2014/15/B/NZ9/01986 dedicated to A.S.).
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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