Furcellaran-Coated Microcapsules as Carriers of Cyprinus carpio Skin-Derived Antioxidant Hydrolysate: An In Vitro and In Vivo Study

Joanna Tkaczewska , Ewelina Jamróz , Ewa Piątkowska , Barbara Borczak , Joanna Kapusta-Duch , Małgorzata Morawska

Abstract

Carp skin gelatine hydrolysate (CSGH) may be a possible bioactive peptide source, as promising antioxidant properties have been noted during in vivo testing. Hence, the present study focused on improving the bioavailability of the antioxidant peptides from CSGH and on the use of furcellaran (FUR), which can protect the biopeptides during digestion in the gastrointestinal tract. Therefore, in this study, microcapsules coated with furcellaran and containing CSGH cores were prepared. The structural properties of the sample were determined using FT-IR and SEM analysis. The antioxidant properties of hydrolysate, uncoated, and encapsulated samples were investigated. In vivo analysis included determination of its safety in an animal organism and evaluation of the lipid profile, antioxidant blood status, and mRNA expression of some genes involved in antioxidant status in Wistar rats. The results showed no adverse effects of microencapsulated protein hydrolysates in laboratory animals. Nonetheless, there was a statistically significant rise in the level of total antioxidant status blood serum among animals consuming CSGH and not inducing oxidative stress. This can be viewed as a promising indication of the positive effects of antioxidant properties tested in vivo. The process of CSGH microencapsulation in FUR cause a decrease in antioxidant hydrolysate activity, both in vitro, as well as in healthy Wistar rats. When considering the results of the presented diverse therapeutic potential, further research on CSGH being a potential bioactive peptide source used as a functional food or nutraceutical, but with a different microencapsulation coating, is encouraged.
Author Joanna Tkaczewska (FoFT / DoAPT)
Joanna Tkaczewska,,
- Department of Animal Product Technology
, Ewelina Jamróz (FoFT / IoCh)
Ewelina Jamróz,,
- Institute of chemistry
, Ewa Piątkowska (FoFT / DoHN)
Ewa Piątkowska,,
- Department of Human Nutrition
, Barbara Borczak (FoFT / DoHN)
Barbara Borczak,,
- Department of Human Nutrition
, Joanna Kapusta-Duch (FoFT / DoHN)
Joanna Kapusta-Duch,,
- Department of Human Nutrition
, Małgorzata Morawska
Małgorzata Morawska,,
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Journal seriesNutrients, ISSN 2072-6643, (N/A 140 pkt)
Issue year2019
Vol11
No10
Pages1-15
Publication size in sheets0.7
Article number2502
Keywords in Englishcarp; gelatine; animal study; antioxidant activity; microcapsules; furcellaran
ASJC Classification1106 Food Science
DOIDOI:10.3390/nu11102502
URL https://www.mdpi.com/2072-6643/11/10/2502/pdf
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
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Furcellaran-Coated Microcapsules as Carriers of Cyprinus carpio Skin-Derived Antioxidant Hydrolysate: An In Vitro and In Vivo Study of 18-10-2019
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Score (nominal)140
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.392; WoS Impact Factor: 2018 = 4.171 (2) - 2018=4.813 (5)
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FinansowanieThis work was supported by the National Centre for Research and Development, Poland (Grant No. Lider/21/0003/l–7/15/NCBR/2016).
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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