Involvement of orexin A in nocturnal melatonin secretion into the cerebrospinal fluid and the blood plasma in seasonal sheep

Katarzyna Kirsz , Małgorzata Szczęsna , Weronika Biernat , Edyta Molik , Dorota Zięba-Przybylska

Abstract

In sheep, differences in orexin A (OXA) gene expression and activity are related to changes in energy demandand seasonal reproduction. However, the mechanism by which and the key place where the OXA signal isintegrated with photoperiod, whose main biochemical expression is melatonin (MEL), remain unknown. Weexamined the effects of cisterna magna injections of OXA (0.3μg/kg body weight) on nocturnal cerebrospinalfluid (CSF) and plasma MEL concentrations; mRNA and protein expression of two rate-limiting enzymes for MELbiosynthesis, tryptophan 5-hydroxylase-1 (TPH1) and arylalkylamine-N-acetyltransferase (AA-NAT); and OXAreceptor (OX1R, OX2R) expression in the pineal gland (PG) obtained from twenty ewes during the short-day (SD)and long-day (LD) seasons. OXA increased (P < 0.001) CSF and plasma MEL concentrations regardless of theseason. Plasma MEL was positively correlated (P < 0.001) with CSF MEL in the OXA-treated sheep in bothseasons. OXA had no effect (P > 0.05) on TPH1 transcript or protein level but upregulated (P < 0.05) AA-NATmRNA and protein expression in both seasons. OXA enhanced (P < 0.05) OX1R mRNA level only during the LDseason. Our results show that the endocrine activity of the ovine PG is regulated by day length and non-photicsignals via hypothalamic OXA. These results are important for understanding the work of the biological clockand recognizing mechanisms responsible for the adaptation of seasonal animals to the changing external en-vironment conditions. OXA and MEL are both involved in the regulation of the sleep-wakefulness system,therefore our results can be used in the study on the circadian rhythm disorders in humans (e.g. jet lag, insomnia,seasonal depression).
Author Katarzyna Kirsz (FoAS / Department of Nutrition, Animal Biotechnology and Fisheries)
Katarzyna Kirsz,,
- Department of Nutrition, Animal Biotechnology and Fisheries
, Małgorzata Szczęsna (FoAS / Department of Nutrition, Animal Biotechnology and Fisheries)
Małgorzata Szczęsna,,
- Department of Nutrition, Animal Biotechnology and Fisheries
, Weronika Biernat (FoAS / Department of Nutrition, Animal Biotechnology and Fisheries)
Weronika Biernat,,
- Department of Nutrition, Animal Biotechnology and Fisheries
, Edyta Molik (FoAS / Department of Nutrition, Animal Biotechnology and Fisheries)
Edyta Molik,,
- Department of Nutrition, Animal Biotechnology and Fisheries
, Dorota Zięba-Przybylska (FoAS / Department of Nutrition, Animal Biotechnology and Fisheries)
Dorota Zięba-Przybylska,,
- Department of Nutrition, Animal Biotechnology and Fisheries
Journal seriesGeneral and Comparative Endocrinology, ISSN 0016-6480, e-ISSN 1095-6840, (N/A 100 pkt)
Issue year2020
Vol286
Pages1-9
Publication size in sheets0.5
Article number113304
Keywords in Englishorexin A, Orexin receptors, Melatonin, Tryptophan 5-hydroxylase-1, Arylalkylamine-N-acetyltransferase, Sheep
ASJC Classification1310 Endocrinology
DOIDOI:10.1016/j.ygcen.2019.113304
URL https://www.sciencedirect.com/science/article/pii/S0016648019302710/pdfft?md5=5ba72540e3873a61e65d345f3fe1d387&pid=1-s2.0-S0016648019302710-main.pdf
Languageen angielski
Score (nominal)100
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.006; WoS Impact Factor: 2018 = 2.445 (2) - 2018=2.66 (5)
Citation count*
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FinansowanieThis work was supported by a grant from the National Science Center in Poland (grant number NCN 2017/01/X/NZ9/00204).
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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