Can Ceylon Leadwort (Plumbago zeylanica L.) Acclimate to Lead Toxicity? -Studies of Photosynthetic Apparatus Efficiency

Krzysztof Tokarz , Wojciech Makowski , Barbara Tokarz , Monika Hanula , Ewa Sitek , Ewa Muszyńska , Roman Jędrzejczyk , Rafał Banasiuk , Łukasz Chajec , Stanisław Mazur

Abstract

Ceylon leadwort (Plumbago zeylanica) is ornamental plant known for its pharmacological properties arising from the abundant production of various secondary metabolites. It often grows in lead polluted areas. The aim of presented study was to evaluate the survival strategy of P. zeylanica to lead toxicity via photosynthetic apparatus acclimatization. Shoots of P. zeylanica were cultivated on media with different Pb concentrations (0.0, 0.05, and 0.1 g Pb∙l−1). After a four-week culture, the efficiency of the photosynthetic apparatus of plants was evaluated by Chl a fluorescence measurement, photosynthetic pigment, and Lhcb1, PsbA, PsbO, and RuBisCo protein accumulation, antioxidant enzymes activity, and chloroplast ultrastructure observation. Plants from lower Pb concentration revealed no changes in photosynthetic pigments content and light-harvesting complex (LHCII) size, as well as no limitation on the donor side of Photosystem II Reaction Centre (PSII RC). However, the activity and content of antioxidant enzymes indicated a high risk of limitation on the acceptor side of Photosystem I. In turn, plants from 0.1 g Pb∙l−1 showed a significant decrease in pigments content, LHCII size, the amount of active PSII RC, oxygen-evolving complex activity, and significant remodeling of chloroplast ultrastructure indicated limitation of PSII RC donor side. Obtained results indicate that P. zeylanica plants acclimate to lead toxicity by Pb accumulation in roots and, depending on Pb concentration, by adjusting their photosynthetic apparatus via the activation of alternative (cyclic and pseudocyclic) electron transport pathways.
Author Krzysztof Tokarz (FoBaH / IoPBaB / DoBaPP)
Krzysztof Tokarz,,
- Department of Botany and Plant Physiology
, Wojciech Makowski (FoBaH)
Wojciech Makowski,,
- Faculty of Biotechnology and Horticulture
, Barbara Tokarz (FoBaH / IoPBaB / DoBaPP)
Barbara Tokarz,,
- Department of Botany and Plant Physiology
, Monika Hanula
Monika Hanula,,
-
, Ewa Sitek (FoBaH / IoPBaB / DoBaPP)
Ewa Sitek,,
- Department of Botany and Plant Physiology
, Ewa Muszyńska
Ewa Muszyńska,,
-
, Roman Jędrzejczyk
Roman Jędrzejczyk,,
-
, Rafał Banasiuk
Rafał Banasiuk,,
-
, Łukasz Chajec
Łukasz Chajec,,
-
, Stanisław Mazur (FoBaH / DoPPro)
Stanisław Mazur,,
- Department of Plant Protection
Journal seriesInternational Journal of Molecular Sciences, ISSN 1422-0067, (N/A 140 pkt)
Issue year2020
Vol21
No5
Pages1-22
Publication size in sheets1.05
Keywords in Englishalternative electron transport; antioxidant enzymes; Chl fluorescence; heavy metals; photosynthesis; secondary metabolites
ASJC Classification2700 General Medicine; 1312 Molecular Biology; 1503 Catalysis; 1604 Inorganic Chemistry; 1605 Organic Chemistry; 1606 Physical and Theoretical Chemistry; 1607 Spectroscopy; 1706 Computer Science Applications
DOIDOI:10.3390/ijms21051866
URL https://www.mdpi.com/1422-0067/21/5/1866
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
File
2020.03.09-tokarz-can-ceylon-leadwort-plumbago-zeylanica-l.acclimate-to-lead-toxicity.pdf 2,31 MB
Score (nominal)140
Score sourcejournalList
Publication indicators WoS Citations = 1; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.224; WoS Impact Factor: 2018 = 4.183 (2) - 2018=4.331 (5)
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