Can Ceylon Leadwort (Plumbago zeylanica L.) Acclimate to Lead Toxicity? -Studies of Photosynthetic Apparatus Efficiency
Krzysztof Tokarz , Wojciech Makowski , Barbara Tokarz , Monika Hanula , Ewa Sitek , Ewa Muszyńska , Roman Jędrzejczyk , Rafał Banasiuk , Łukasz Chajec , Stanisław Mazur
AbstractCeylon leadwort (Plumbago zeylanica) is ornamental plant known for its pharmacological properties arising from the abundant production of various secondary metabolites. It often grows in lead polluted areas. The aim of presented study was to evaluate the survival strategy of P. zeylanica to lead toxicity via photosynthetic apparatus acclimatization. Shoots of P. zeylanica were cultivated on media with different Pb concentrations (0.0, 0.05, and 0.1 g Pb∙l−1). After a four-week culture, the efficiency of the photosynthetic apparatus of plants was evaluated by Chl a fluorescence measurement, photosynthetic pigment, and Lhcb1, PsbA, PsbO, and RuBisCo protein accumulation, antioxidant enzymes activity, and chloroplast ultrastructure observation. Plants from lower Pb concentration revealed no changes in photosynthetic pigments content and light-harvesting complex (LHCII) size, as well as no limitation on the donor side of Photosystem II Reaction Centre (PSII RC). However, the activity and content of antioxidant enzymes indicated a high risk of limitation on the acceptor side of Photosystem I. In turn, plants from 0.1 g Pb∙l−1 showed a significant decrease in pigments content, LHCII size, the amount of active PSII RC, oxygen-evolving complex activity, and significant remodeling of chloroplast ultrastructure indicated limitation of PSII RC donor side. Obtained results indicate that P. zeylanica plants acclimate to lead toxicity by Pb accumulation in roots and, depending on Pb concentration, by adjusting their photosynthetic apparatus via the activation of alternative (cyclic and pseudocyclic) electron transport pathways.
|Journal series||International Journal of Molecular Sciences, ISSN 1422-0067, (N/A 140 pkt)|
|Publication size in sheets||1.05|
|Keywords in English||alternative electron transport; antioxidant enzymes; Chl fluorescence; heavy metals; photosynthesis; secondary metabolites|
|ASJC Classification||; ; ; ; ; ; ;|
|License||Journal (articles only); author's original; ; after publication|
|Publication indicators||= 1; : 2018 = 1.224; : 2018 = 4.183 (2) - 2018=4.331 (5)|
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