Cytogenetic karyotype analysis in selected species of the Erinaceidae family

Anna Grzesiakowska , Przemysław Baran , Marta Kuchta-Gładysz , Olga Szeleszczuk

Abstract

Introduction: Comparative analysis of the karyotype structure was made in two hedgehog species: the northern white-breasted hedgehog (Erinaceus roumanicus) and the African pygmy hedgehog (Atelerix albiventris). Material and Methods: The cytogenetic analysis used differential staining techniques (DAPI, Ag-NOR, and C-banding/DAPI) and sequential QFQ/FISH banding with NOR20 and TEL20 probes which showed 45S rDNA and (TTAGGG)n repeat sequences, respectively, on hedgehog chromosomes. Results: It was confirmed that the somatic cells of the hedgehogs have a constant number of chromosomes (2n = 48,XY). Differences were observed in the NOR number between the species. NORs were identified on three autosome pairs in the northern white-breasted hedgehog and on only two pairs in the African pygmy hedgehog. Chromosome analysis by C-banding/DAPI showed large segments of heterochromatin rich in A-T pairs on three autosome pairs in both the northern white-breasted and African pygmy hedgehogs. The heterochromatin segments encompassed large fragments of the longer arm of chromosome pairs 13, 14 and 20. The (TTAGGG)n repeat sequences on the hedgehog chromosomes were only observed in the terminal position of all the chromosomes in both species. Conclusion: Our observations provide new information on the level of diversity within the Erinaceidae family.
Author Anna Grzesiakowska (FoAS / IoVS)
Anna Grzesiakowska,,
- Institute of Veterinary Sciences
, Przemysław Baran
Przemysław Baran,,
-
, Marta Kuchta-Gładysz (FoAS / IoVS)
Marta Kuchta-Gładysz,,
- Institute of Veterinary Sciences
, Olga Szeleszczuk (FoAS / IoVS)
Olga Szeleszczuk,,
- Institute of Veterinary Sciences
Journal seriesJournal of Veterinary Research, [Bulletin of the Veterinary Institute in Pulawy, Journal of Veterinary Research (Poland)], ISSN 2450-7393, e-ISSN 2450-8608, (N/A 40 pkt)
Issue year2019
Vol63
Pages353-358
Publication size in sheets0.5
Keywords in Englishhedgehog; chromosomes; heterochromatin; nucleolar organisers; telomere
ASJC Classification3400 General Veterinary
DOIDOI:10.2478/jvetres-2019-0041
URL https://content.sciendo.com/downloadpdf/journals/jvetres/ahead-of-print/article-10.2478-jvetres-2019-0041.xml
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa - Użycie Niekomercyjne - Bez utworów zależnych (CC-BY-NC-ND); after publication
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Cytogenetic karyotype analysis in selected species of the Erinaceidae family 672,53 KB
Score (nominal)40
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.501; WoS Impact Factor: 2018 = 0.829 (2) - 2018=0.938 (5)
Citation count*
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FinansowanieThe study was supported by the Mission-Related Research project DS-3252/ZAZ of the University of Agriculture in Kraków.
Udział procentowyA. Grzesiakowska: 50%, P. Baran: 0%, M. Kuchta-Gladysz: 40%, O. Szeleszczuk: 10%
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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