Annual Reproductive Performance of Eisenia andrei and E. fetida 2 in Intra- and Inter-Specific Pairs and Lack of Reproduction of Isolated Virgin Earthworms

Agnieszka Podolak , Joanna Kostecka , Sebastian Hofman , Artur Osikowski , Janusz Bigaj , Barbara Płytycz

Abstract

Eisenia andrei/fetida complex of lumbricid earthworms contains E. andrei (Ea) and two mitochondrial lineages of E. fetida (Ef), referred to as Ef1 and Ef2. These earthworms are hermaphrodites capable of self-fertilization and hybridization as evidenced in laboratory mated earthworms from Ea and Ef1 lineage of Ef. The aim of the present investigations was to compare reproductive performance of Ea and Ef2 lineage from French laboratory stocks reared for a decade in Polish laboratories. These were cultured either in isolation and/or in intra-specific or inter-specific pairs for up to 57 weeks from hatching. Parental specimens and offspring were identified by species/lineage-specific sequences of the haploid mitochondrial COI gene, either ‘a’ or ‘f2’, and species-specific sequences of the nuclear 28s rRNA gene, either ‘AA’ or ‘FF’, thus delimitated as aAA or f2FF for Ea or Ef2, respectively, or aAF for hybrids. Isolated virgin earthworms produced a few sterile cocoons only, more frequently in Ef2 than in Ea, but no hatchlings. Analysis of cocoon production and reproduction of laboratory-mated intra-specific Ea+Ea and Ef2+Ef2 pairs revealed higher fecundity of Ea than Ef2 measured by numbers of cocoons and hatchlings, while inter-specific Ea+Ef2 pairs gave plenty cocoons but low numbers of aAF hybrids developed from Ea ova fertilized by Ef2 spermatozoa.
Author Agnieszka Podolak - [Jagiellonian University (UJ)]
Agnieszka Podolak,,
-
- Uniwersytet Jagielloński w Krakowie
, Joanna Kostecka - [Uniwersytet Rzeszowski]
Joanna Kostecka,,
-
- Uniwersytet Rzeszowski
, Sebastian Hofman - [Jagiellonian University (UJ)]
Sebastian Hofman,,
-
- Uniwersytet Jagielloński w Krakowie
, Artur Osikowski (FoAS / Department of Animal Reproduction, Anatomy and Genomics)
Artur Osikowski,,
- Department of Animal Reproduction, Anatomy and Genomics
, Janusz Bigaj - [Jagiellonian University (UJ)]
Janusz Bigaj,,
-
- Uniwersytet Jagielloński w Krakowie
, Barbara Płytycz - [Jagiellonian University (UJ)]
Barbara Płytycz,,
-
- Uniwersytet Jagielloński w Krakowie
Journal seriesFolia Biologica-Krakow, [Folia Biologica], ISSN 0015-5497, e-ISSN 1734-9168, (N/A 40 pkt)
Issue year2020
Vol68
No1
Pages1-6
Publication size in sheets0.5
Keywords in Englishlumbricid earthworms, cocoons, hatchlings, hybridization, self-fertilization
ASJC Classification2700 General Medicine; 1300 General Biochemistry, Genetics and Molecular Biology
DOIDOI:10.3409/fb_68-1.01
URL http://www.isez.pan.krakow.pl/journals/folia/pdf/68(1)/68(1)_01.pdf
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
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Annual Reproductive Performance of Eisenia andrei and E. fetida 2 in Intra- and Inter-Specific Pairs and Lack of Reproduction of Isolated Virgin Earthworms of 12-05-2020
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Score (nominal)40
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.483; WoS Impact Factor: 2018 = 0.724 (2) - 2018=0.628 (5)
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