The role of chloroplasts in the oxidative stress that is induced by zearalenone in wheat plants – The functions of 24-epibrassinolide and selenium in the protective mechanisms

Maria Filek , Apolonia Sieprawska , Janusz Kościelniak , Jana Oklešťková , Barbara Jurczyk , Anna Telk , Jolanta Biesaga-Kościelniak , Anna Janeczko

Abstract

This study focused on the idea that the toxic effect of zearalenone (ZEA) and the protective actions of the brassinosteroid - 24-epibrassinolide (EBR) as well as selenium are dependent on its accumulation in chloroplasts to a high degree. These organelles were isolated from the leaves of oxidative stress-sensitive and stress-tolerant wheat cultivars that had been grown from grains that had been incubated in a solution of ZEA (30 μM), Na2SeO4 (Se, 10 μM), EBR (0.1 μM) or in a mixture of ZEA with Se or EBR. Ultra-high performance liquid chromatography techniques indicated that ZEA was adsorbed in higher amounts in the chloroplasts in the sensitive rather than tolerant cultivar. Although the brassinosteroids and Se were also accumulated in the chloroplasts, higher levels were only found in the tolerant cultivar. The application of EBR increased the homocastasterone content, especially in the chloroplasts of the tolerant plant and after the addition of ZEA. The presence of both protectants caused a decrease in the ZEA content in studied organelles and resulted in diminishing of the oxidative stress (i.e. changes in the activity of the antioxidative enzymes). Moreover, a recovery of photosystem II and decrease in the negative impact of ZEN on Hsp90 transcript accumulation was observed in plants.
Author Maria Filek
Maria Filek,,
-
, Apolonia Sieprawska
Apolonia Sieprawska,,
-
, Janusz Kościelniak (FoAE / DoPP)
Janusz Kościelniak,,
- Department of Plant Physiology
, Jana Oklešťková
Jana Oklešťková,,
-
, Barbara Jurczyk (FoAE / DoPP)
Barbara Jurczyk,,
- Department of Plant Physiology
, Anna Telk
Anna Telk,,
-
, Jolanta Biesaga-Kościelniak
Jolanta Biesaga-Kościelniak,,
-
, Anna Janeczko
Anna Janeczko,,
-
Journal seriesPlant Physiology and Biochemistry, ISSN 0981-9428, (N/A 70 pkt)
Issue year2019
No137
Pages84-92
Publication size in sheets0.5
Keywords in EnglishWheat, Chloroplasts, Zearalenone, Brassinosteroids, Selenium, Photosynthesis, Hsp90
ASJC Classification1110 Plant Science; 1311 Genetics; 1314 Physiology
DOIDOI:10.1016/j.plaphy.2019.01.030
URL https://www.sciencedirect.com/science/article/pii/S0981942819300385?via%3Dihub
Internal identifierWRE/2019/61
Languageen angielski
Score (nominal)70
Score sourcejournalList
Publication indicators WoS Citations = 1; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.092; WoS Impact Factor: 2018 = 3.404 (2) - 2018=3.607 (5)
Citation count*
Additional fields
FinansowanieThis work was supported by a project of National Science Centre (Poland) No. 2014/15/B/NZ9/02192. The authors thank Dr. Ondrej Novák (Laboratory of Growth Regulation, Olomouc, Czech Republic) for his technical assistance in UHPLC-MS/MS measurements of the brassinosteroids.
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back
Confirmation
Are you sure?