Spontaneous DNA Damage in Lymphocytes of Fox Species
Anna Grzesiakowska , Olga Szeleszczuk , Marta Kuchta-Gładysz , Agnieszka Otwinowska-Mindur
AbstractThe comet assay is a common tool used to evaluate cellular genetic instability at the DNA level. It determines the effect of physical, chemical and environmental factors on DNA, and thus shows the body’s individual resistance to harmful substances. The karyotypes of blue and silver foxes and their inter species hybrids are characterized by morphological and structural variation. This variation is partly attributable to the presence of chromosomal polymorphism, which may significantly influence the stability of genetic material in the cells of these species. The aim of this study was to evaluate genetic material stability in selected Canidae species. To this end, analyses using the alkaline comet assay were performed. The interaction between sex and species was significant (p<0.05) for percentage of head DNA, tail DNA and Olive Tail Moment, and highly significant (p<0.01) for tail length and Tail Moment. The chromosomal polymorphism observed in the somatic cells of original species (blue and silver fox), does not affect the stability of the genetic material in the interspecific fox hybrids.
|Journal series||Folia Biologica-Krakow, [Folia Biologica], ISSN 0015-5497, e-ISSN 1734-9168, (N/A 40 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Foxes, comet assay, DNA integrity|
|License||Journal (articles only); author's original; ; after publication|
|Publication indicators||= 0; : 2018 = 0.483; : 2018 = 0.724 (2) - 2018=0.628 (5)|
|Finansowanie||This study was financed as part of the Statutory Activity of the Department of Animal Anatomy, DS-3253/ZAZ of the University of Agriculture in Krakow.|
|Udział procentowy||Anna Grzesiakowska: 45%, Olga Szeleszczuk: 10%, Marta Kuchta-Gładysz: 35%, Agnieszka Otwinowska-Mindur: 10%|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.