Spontaneous DNA Damage in Lymphocytes of Fox Species

Anna Grzesiakowska , Olga Szeleszczuk , Marta Kuchta-Gładysz , Agnieszka Otwinowska-Mindur

Abstract

The comet assay is a common tool used to evaluate cellular genetic instability at the DNA level. It determines the effect of physical, chemical and environmental factors on DNA, and thus shows the body’s individual resistance to harmful substances. The karyotypes of blue and silver foxes and their inter species hybrids are characterized by morphological and structural variation. This variation is partly attributable to the presence of chromosomal polymorphism, which may significantly influence the stability of genetic material in the cells of these species. The aim of this study was to evaluate genetic material stability in selected Canidae species. To this end, analyses using the alkaline comet assay were performed. The interaction between sex and species was significant (p<0.05) for percentage of head DNA, tail DNA and Olive Tail Moment, and highly significant (p<0.01) for tail length and Tail Moment. The chromosomal polymorphism observed in the somatic cells of original species (blue and silver fox), does not affect the stability of the genetic material in the interspecific fox hybrids.
Author Anna Grzesiakowska (FoAS / IoVS)
Anna Grzesiakowska,,
- Institute of Veterinary Sciences
, Olga Szeleszczuk (FoAS / IoVS)
Olga Szeleszczuk,,
- Institute of Veterinary Sciences
, Marta Kuchta-Gładysz (FoAS / IoVS)
Marta Kuchta-Gładysz,,
- Institute of Veterinary Sciences
, Agnieszka Otwinowska-Mindur (DoAS / DoGaAIM)
Agnieszka Otwinowska-Mindur,,
- Department of Genetics and Animal Improvement Methods
Journal seriesFolia Biologica-Krakow, [Folia Biologica], ISSN 0015-5497, e-ISSN 1734-9168, (N/A 40 pkt)
Issue year2019
Vol67
No1
Pages1-7
Publication size in sheets0.5
Keywords in EnglishFoxes, comet assay, DNA integrity
ASJC Classification2700 General Medicine; 1300 General Biochemistry, Genetics and Molecular Biology
DOIDOI:10.3409/fb_67-1.01
URL http://www.isez.pan.krakow.pl/journals/folia/pdf/67(1)/67(1)_01.pdf
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
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Spontaneous DNA Damage in Lymphocytes of Fox Species of 23-07-2019
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Score (nominal)40
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.483; WoS Impact Factor: 2018 = 0.724 (2) - 2018=0.628 (5)
Citation count*
Additional fields
FinansowanieThis study was financed as part of the Statutory Activity of the Department of Animal Anatomy, DS-3253/ZAZ of the University of Agriculture in Krakow.
Udział procentowyAnna Grzesiakowska: 45%, Olga Szeleszczuk: 10%, Marta Kuchta-Gładysz: 35%, Agnieszka Otwinowska-Mindur: 10%
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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