Exogenous melatonin stimulated Amaryllidaceae alkaloid biosynthesis in in vitro cultures of Leucojum aestivum L

Agata Ptak , Magdalena Simlat , Emilia Morańska , Edyta Skrzypek , Marzena Warchoł , Ameneh Tarakemeh , Dominique Laurain-Mattar

Abstract

Leucojum aestivum L. is a highly demanding pharmaceutical plant which is a natural source of pharmacologically active Amaryllidaceae alkaloids, especially galanthamine and lycorine. To establish an efficient method for the production of these alkaloids Leucojum aestivum plants derived from somatic embryos were subjected to various concentrations of melatonin (N-acetyl-5-methoxy tryptamine), NaCl and melatonin combined with NaCl. The maximum L. aestivum plant biomass was obtained when 10 μM of melatonin was applied to the medium. These plants were characterised by normal growth and did not reveal signs of browning. Enrichment of the medium with NaCl caused a reduction in plant biomass, plant pigment contents (chlorophyll a, chlorophyll b and carotenoids) and an increase in the activities of antioxidant enzymes catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD). The addition of melatonin to the medium containing NaCl neutralised the negative effect of salt stress. Moreover, the stimulating effect of melatonin on Amaryllidaceae alkaloids biosynthesis was observed. In plants grown in the medium enriched with melatonin the galanthamine content was, on average, 58.6 times higher, and the lycorine content was 1.5 times higher as compared with the control. In vitro plants obtained from the medium supplemented with melatonin also showed the highest distribution of alkaloids. It is noteworthy that only the plants grown in the medium with melatonin showed the presence of tazettine and chlidanthine, 1,2-dihydro-. This study presents a promising biotechnological approach for the commercial production of alkaloids, including galanthamine and lycorine.
Author Agata Ptak (FoAE / DoPBaS)
Agata Ptak,,
- Department of Plant Breeding and Seed Science
, Magdalena Simlat (FoAE / DoPBaS)
Magdalena Simlat,,
- Department of Plant Breeding and Seed Science
, Emilia Morańska (FoAE / DoPBaS)
Emilia Morańska,,
- Department of Plant Breeding and Seed Science
, Edyta Skrzypek
Edyta Skrzypek,,
-
, Marzena Warchoł
Marzena Warchoł,,
-
, Ameneh Tarakemeh
Ameneh Tarakemeh,,
-
, Dominique Laurain-Mattar
Dominique Laurain-Mattar,,
-
Journal seriesIndustrial Crops and Products, ISSN 0926-6690, e-ISSN 1872-633X, (N/A 200 pkt)
Issue year2019
Vol138
Pages1-8
Publication size in sheets0.5
Article number111458
Keywords in EnglishLeucojum aestivum; In vitro plants; Melatonin; NaCl; Galanthamine; Lycorine
ASJC Classification1102 Agronomy and Crop Science
DOIDOI:10.1016/j.indcrop.2019.06.021
URL https://www.sciencedirect.com/science/article/abs/pii/S0926669019304613
Languageen angielski
Score (nominal)200
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.693; WoS Impact Factor: 2018 = 4.191 (2) - 2018=4.419 (5)
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