Selenium and Zinc Biofortification of Pleurotus eryngii Mycelium and Fruiting Bodies as a Tool for Controlling Their Biological Activity

Piotr Zięba , Katarzyna Kała , Anna Włodarczyk , Agnieszka Szewczyk , Edward Kunicki , Agnieszka Sękara , Bożena Muszyńska

Abstract

Pleurotus eryngii (DC:Fr.) Quel. is a cultivated mushroom of high culinary value and medicinal properties. Mycelium of P.eryngii is characterized by the ability of effective bio-elements absorptionfromgrowthmediasoitcouldbebiofortifiedwithtraceelementswithafunctionalactivity in the human body. In this study, the ability of P. eryngii mycelia from in vitro cultures as well as fruiting bodies were investigated in terms of their effectiveness in zinc and selenium accumulation. The effect of Se and Zn biofortification on productivity, chemical compounds, and bio-elements content of P. eryngii was determined as well. To enhance Se and Zn content in P. eryngii fruiting bodies and mycelia, substrates were supplemented with sodium selenite, at a concentration of 50 mg L−1, zinc sulfate, and zinc hydro-aspartate at a concentration of 87.2 and 100.0 mg L−1, respectively. Mentioned Zn concentrations contained the same amount of zinc(II) ions, namely 20mgL−1. Thecontentoforganiccompoundsincludephenoliccompoundsandlovastatin,whichwere determined by a high-performance liquid chromatography with diode-array detector (HPLC-DAD) and reverse phase high-performance liquid chromatography (RP-HPLC) method with UV detection. The ability of P. eryngii to accumulate zinc and selenium from the culture medium was demonstrated. The degree of accumulation of zinc turned out to be different depending on the type of salt used. ThepresentstudyalsoshowedthatconductingmyceliumofP.eryngiiinin vitroculture,withahigher contentofzincions,canresultinobtainingthematerialswithbetterantioxidantability. Theresultsof this study can be used to develop the composition of growing media, which ensures the production of biomass with the desired composition of elements.
Author Piotr Zięba (FoBaH / DoVaMP)
Piotr Zięba,,
- Department of Vegetable and Medicinal Plants
, Katarzyna Kała
Katarzyna Kała,,
-
, Anna Włodarczyk
Anna Włodarczyk,,
-
, Agnieszka Szewczyk
Agnieszka Szewczyk,,
-
, Edward Kunicki (FoBaH / DoVaMP)
Edward Kunicki,,
- Department of Vegetable and Medicinal Plants
, Agnieszka Sękara (FoBaH / DoVaMP)
Agnieszka Sękara,,
- Department of Vegetable and Medicinal Plants
, Bożena Muszyńska
Bożena Muszyńska,,
-
Journal seriesMolecules, ISSN 1420-3049, (N/A 100 pkt)
Issue year2020
Vol25
No4
Pages1-18
Publication size in sheets0.85
Article number889
Keywords in Englishbiofortification; lovastatin; phenoloic compounds; Pleurotus eryngii; selenium; zinc
ASJC Classification1605 Organic Chemistry
DOIDOI:10.3390/molecules25040889
URL https://www.mdpi.com/1420-3049/25/4/889/pdf
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
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Selenium and Zinc Biofortification of Pleurotus eryngii Mycelium and Fruiting Bodies as a Tool for Controlling Their Biological Activity of 17-03-2020
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Score (nominal)100
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.146; WoS Impact Factor: 2018 = 3.06 (2) - 2018=3.38 (5)
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FinansowanieThe Ministry of Science and Higher Education of the Republic of Poland partially supported this work.
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