Selenium and Zinc Biofortification of Pleurotus eryngii Mycelium and Fruiting Bodies as a Tool for Controlling Their Biological Activity
Piotr Zięba , Katarzyna Kała , Anna Włodarczyk , Agnieszka Szewczyk , Edward Kunicki , Agnieszka Sękara , Bożena Muszyńska
AbstractPleurotus eryngii (DC:Fr.) Quel. is a cultivated mushroom of high culinary value and medicinal properties. Mycelium of P.eryngii is characterized by the ability of eﬀective bio-elements absorptionfromgrowthmediasoitcouldbebiofortiﬁedwithtraceelementswithafunctionalactivity in the human body. In this study, the ability of P. eryngii mycelia from in vitro cultures as well as fruiting bodies were investigated in terms of their eﬀectiveness in zinc and selenium accumulation. The eﬀect of Se and Zn biofortiﬁcation on productivity, chemical compounds, and bio-elements content of P. eryngii was determined as well. To enhance Se and Zn content in P. eryngii fruiting bodies and mycelia, substrates were supplemented with sodium selenite, at a concentration of 50 mg L−1, zinc sulfate, and zinc hydro-aspartate at a concentration of 87.2 and 100.0 mg L−1, respectively. Mentioned Zn concentrations contained the same amount of zinc(II) ions, namely 20mgL−1. Thecontentoforganiccompoundsincludephenoliccompoundsandlovastatin,whichwere determined by a high-performance liquid chromatography with diode-array detector (HPLC-DAD) and reverse phase high-performance liquid chromatography (RP-HPLC) method with UV detection. The ability of P. eryngii to accumulate zinc and selenium from the culture medium was demonstrated. The degree of accumulation of zinc turned out to be diﬀerent depending on the type of salt used. ThepresentstudyalsoshowedthatconductingmyceliumofP.eryngiiinin vitroculture,withahigher contentofzincions,canresultinobtainingthematerialswithbetterantioxidantability. Theresultsof this study can be used to develop the composition of growing media, which ensures the production of biomass with the desired composition of elements.
|Journal series||Molecules, ISSN 1420-3049, (N/A 100 pkt)|
|Publication size in sheets||0.85|
|Keywords in English||biofortiﬁcation; lovastatin; phenoloic compounds; Pleurotus eryngii; selenium; zinc|
|License||Journal (articles only); author's original; ; after publication|
|Publication indicators||= 0; : 2017 = 1.146; : 2018 = 3.06 (2) - 2018=3.38 (5)|
|Finansowanie||The Ministry of Science and Higher Education of the Republic of Poland partially supported this work.|
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