Pregnancy‐induced changes in the transcript levels of prolactin receptor and its suppressor in the ovine hypothalamus and adenohypophysis

Małgorzata Szczęsna , Katarzyna Kirsz , Tomasz Misztal , Dorota Zięba-Przybylska

Abstract

The aim of this study was to analyse changes in the abundance of prolactin (PRL) receptor (PRLR) and suppressor of cytokine signalling‐3 (SOCS‐3) mRNA in the ventro‐/dorsomedial nucleus (VMH/DMH) and arcuate nucleus (ARC) of the hypothalamus as well as in the median eminence (ME) and adenohypophysis (AP) in sheep at 30, 60, 90 and 120 d of pregnancy compared to non‐pregnant animals. In the VMH/DMH, PRLR transcripts were detected only in non‐pregnant ewes. In the ARC, the abundances of PRLR mRNA were higher in pregnant sheep on days 30 (p < .01), 90 (p < .01) and 120 (p < .05) than in non‐pregnant sheep. In contrast, the expression of PRLR mRNA in the ME was lower (p < .01) in pregnant ewes at days 30 and 60 than in non‐pregnant ewes and was undetectable at later stages of gestation. In all studied stages of pregnancy except day 60, the abundance of PRLR mRNA was higher (p < .01) in the ARC than in the AP, while in non‐pregnant sheep, there were no differences (p ≥ .05) in the transcript levels between these two tissues. In non‐pregnant ewes, the abundance of SOCS‐3 mRNA in the AP was lower than that in any other studied tissue (p < .05‐p < .01). In conclusion, the observed changes in PRLR and SOCS‐3 mRNA abundance in the hypothalamus and AP during pregnancy may be important components of the mechanisms regulating the action of PRL in energy homeostasis and neuroendocrine interactions within the hypothalamic–pituitary axis.
Author Małgorzata Szczęsna (FoAS / DoAB)
Małgorzata Szczęsna,,
- Department of Animal Biotechnology
, Katarzyna Kirsz (FoAS / DoAB)
Katarzyna Kirsz,,
- Department of Animal Biotechnology
, Tomasz Misztal
Tomasz Misztal,,
-
, Dorota Zięba-Przybylska (FoAS / DoAB)
Dorota Zięba-Przybylska,,
- Department of Animal Biotechnology
Journal seriesReproduction in Domestic Animals, ISSN 0936-6768, e-ISSN 1439-0531, (N/A 100 pkt)
Issue year2020
Vol55
No1
Pages21-28
Publication size in sheets0.5
Keywords in Englishpregnancy, prolactin, prolactin receptor, sheep, SOCS‐3
ASJC Classification1103 Animal Science and Zoology; 1305 Biotechnology; 1310 Endocrinology
DOIDOI:10.1111/rda.13576
URL https://onlinelibrary.wiley.com/doi/epdf/10.1111/rda.13576
Languageen angielski
Score (nominal)100
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 0.934; WoS Impact Factor: 2018 = 1.638 (2) - 2018=1.677 (5)
Citation count*
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FinansowanieThis work was supported by the National Science Centre in Poland (NCN 2013/09/B/NZ4/01532). The authors would like to thank Natalia Sowińska, PhD, for providing veterinary care to the experi‐mental animals.
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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