Establishment of an in vitro culture of Pelargonium x domesticum cultivars characterized by different growth requirements : Short communication

Ewa Hanus-Fajerska , Agnieszka Mrzygłód , Alina Wiszniewska , Aleksandra Koźmińska , Piotr Stolarczyk

Abstract

Experiments were carried out to develop micropropagation protocols for cultivars of hybrid origin which have been not cultivated via tissue culture. Proliferating cultures of Pelargonium × domesticum “Tip Top Duet” and “Black Knight” were obtained even if initially the number of excised aseptic nodal pieces was considerably reduced due to contamination with endogenic bacteria. The supplementation of a maintenance medium with either 100 or 200 mg/l peptone proved beneficial. The best propagation coefficient, exceeding 82 adventitious shoots from one initial microcutting, was obtained on a modified MS medium with an addition of 200 mg/l peptone and 0.5 mg/l adenine. The regenerated shoots readily developed new, anatomically properly formed leaves. In the plant material studied, the leaf epidermis produced glandular trichomata which were similar in structure to those observed in a standard cultivar.
Author Ewa Hanus-Fajerska (FoBaH / IoPBaB)
Ewa Hanus-Fajerska,,
- Institute of Plant Biology and Biotechnology
, Agnieszka Mrzygłód (FoBaH / IoPBaB)
Agnieszka Mrzygłód,,
- Institute of Plant Biology and Biotechnology
, Alina Wiszniewska (FoBaH / IoPBaB)
Alina Wiszniewska,,
- Institute of Plant Biology and Biotechnology
, Aleksandra Koźmińska (FoBaH / IoPBaB)
Aleksandra Koźmińska,,
- Institute of Plant Biology and Biotechnology
, Piotr Stolarczyk (FoBaH / IoPBaB)
Piotr Stolarczyk,,
- Institute of Plant Biology and Biotechnology
Journal seriesBioTechnologia Journal of Biotechnology, Computational Biology and Bionanotechnology, ISSN 0860-7796, (B 13 pkt)
Issue year2015
Vol96
No2
Pages203-207
Publication size in sheets0.5
Keywords in Englishmicropropagation, Pelargonium, plant tissue culture, Geraniaceae, peptone
ASJC Classification1110 Plant Science; 1305 Biotechnology
DOIDOI:10.5114/bta.2015.54747
URL http://www.biotechnologia-journal.org/Journal/-85/pdf-25939-10?filename=BTA#215-06-str203-207.pdf
Internal identifierWBIO/2015/65
Languageen angielski
Score (nominal)13
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2015 = 0.274
Citation count*
Additional fields
FinansowanieThis study was financially supported by the Polish Ministry of Science and Higher Education in the form of DS 3500/ZBiFR.
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