Using rapid i method for vitrification of bovine oocytes

Patrycja Mrowiec , Agnieszka Nowak , Joanna Kochan , Wiesława Młodawska

Abstract

BACKGROUND: Vitrification is commonly used for cryopreservation of gametes. OBJECTIVE: The study aimed at evaluating viability and developmental competence of bovine oocytes vitrified by Rapid-I method. MATERIALS AND METHODS: Oocytes after collection (group 1) and IVM (group 2) were vitrified using medium containing 18% Ficoll, 40% ethylene glycol, 0.3 M sucrose. To evaluate viability, oocytes after warming were stained with fluorescein diacetate and ethidium bromide. In experiment 2, oocytes after IVM and vitrification were activated by 0.5 µM ionomycin in TCM 199 combined with 2 mM 6-DMAP in TCM 199 with 10% fetal bovine serum. RESULTS: Survival rate in group 1 was 58%, and 88% in the control. In group 2, 63% viable oocytes were found, compared to 82% in the control group. After parthenogenetic activation 27.2% morulas were observed. This percentage was lower than in the non-vitrified group (31%). CONCLUSION: Maturity stage of bovine oocytes has no effect on their survival after vitrification.
Author Patrycja Mrowiec (FoAS / IoVS)
Patrycja Mrowiec,,
- Institute of Veterinary Sciences
, Agnieszka Nowak (FoAS / IoVS)
Agnieszka Nowak,,
- Institute of Veterinary Sciences
, Joanna Kochan (FoAS / IoVS)
Joanna Kochan,,
- Institute of Veterinary Sciences
, Wiesława Młodawska (FoAS / IoVS)
Wiesława Młodawska,,
- Institute of Veterinary Sciences
Journal seriesCryoletters, [Cryo-Letters], ISSN 0143-2044, e-ISSN 1742-0644, (N/A 40 pkt)
Issue year2019
No40 (2)
Pages123-128
Publication size in sheets0.5
Keywords in Englishbovine, oocyte, Rapid I, vitrification
ASJC Classification2700 General Medicine
URL http://www.cryoletters.org/Abstracts/vol_40_2_2019.htm#123
Internal identifierWHiBZ/2019/28
Languageen angielski
Score (nominal)40
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.37; WoS Impact Factor: 2018 = 0.694 (2) - 2018=0.842 (5)
Citation count*
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back
Confirmation
Are you sure?