Iodine biofortification through expression of HMT, SAMT and S3H genes in Solanum lycopersicum L

Mariya Halka , Sylwester Smoleń , Małgorzata Czernicka , Magdalena Klimek-Chodacka , Joanna Pitala , Krzysztof Tutaj

Abstract

The uptake process and physiological reaction of plants to aromatic iodine compounds have not yet been documented. The aim of this research was to compare uptake by tomato plants of KI and KIO3, as well as of organic iodine compounds – 5-ISA (5-iodosalicylic acid), 3,5-diISA (3,5-diiodosalicylic acid), 2-IBeA (2-iodobenzoic acid), 4-IBeA (4-iodobenzoic acid) and 2,3,5-triIBeA (2,3,5-triiodobenzoic acid). Only 2,3,5-triIBeA had a negative influence on plant development. All organic iodine compounds were taken up by roots and transported to leaves and fruits. Among all the compounds applied, the most efficiently transferred iodine was 2-IBeA – to fruits, and 4-IBeA – to leaves. The order of iodine accumulation in fruit cell compartments was as follows: organelles > cell walls > soluble portions of cells; for leaf and root cells, it was: organelles > cell walls or soluble portions, depending on the compound applied. The compounds studied influence iodine metabolism through expression of the HMT gene which encodes halide ion methyltransferase in leaves and roots. Also, their influence on modification of the activity of the SAMT and S3H genes that encode salicylic acid carboxyl methyltransferase and salicylic acid 3-hydroxylase was established. It was discovered that exogenously applied 5-ISA, 3,5-diISA, 2-IBeA and 4-IBeA are genuinely (endogenously) synthesised in tomato plants; to date, this has not been described for the tomato, nor for any other species of higher plant.
Author Mariya Halka (FoBaH / IoPBaB)
Mariya Halka,,
- Institute of Plant Biology and Biotechnology
, Sylwester Smoleń (FoBaH / IoPBaB)
Sylwester Smoleń,,
- Institute of Plant Biology and Biotechnology
, Małgorzata Czernicka (FoBaH / IoPBaB / DoGPBaSP)
Małgorzata Czernicka,,
- Department of Genetics, Plant Breeding and Seed Production
, Magdalena Klimek-Chodacka (FoBaH / IoPBaB)
Magdalena Klimek-Chodacka,,
- Institute of Plant Biology and Biotechnology
, Joanna Pitala (FoBaH / IoPBaB)
Joanna Pitala,,
- Institute of Plant Biology and Biotechnology
, Krzysztof Tutaj
Krzysztof Tutaj,,
-
Journal seriesPlant Physiology and Biochemistry, ISSN 0981-9428, (N/A 70 pkt)
Issue year2019
Vol144
Pages35-48
Publication size in sheets0.65
Keywords in EnglishBiofortification; Organoiodine compounds; Iodosalicylates; Iodobenzoates; Iodine metabolism; Iodine methylation; Salicylic acid metabolism
ASJC Classification1110 Plant Science; 1311 Genetics; 1314 Physiology
DOIDOI:10.1016/j.plaphy.2019.09.028
URL https://www.sciencedirect.com/science/article/pii/S0981942819303729?via%3Dihub
Languageen angielski
Score (nominal)70
Score sourcejournalList
Publication indicators WoS Citations = 1; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.092; WoS Impact Factor: 2018 = 3.404 (2) - 2018=3.607 (5)
Citation count*
Additional fields
FinansowanieThis research was financed by the Ministry of Science and Higher Education of the Republic of Poland. The method of enriching plants with iodine with: 5-iodosalicylic acid, 3,5-diiodosalicylic acid, 2-iodobenzoic acid, 4-iodobenzoic acid and 2,3,5-triiodobenzoic acid is patented in Polish Patent Office-patent number P.410806 (November 20, 2017).
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back
Confirmation
Are you sure?