The influence of methionine in ovo injection on white blood cells picture of new hatched chicks

K. B. Głodek , A. Ludwikowska , Marcin Lis , Barbara Tombarkiewicz , Krzysztof Pawlak , J. W. Niedziółka

Abstract

The aim of this experiment was to assess the effect of an in ovo injection of methionine in the early stage of embryogenesis on leukocyte blood picture of new hatched chicks. Hatching eggs from parental stock of Ross 308 were incubated under standard conditions. An in ovo injection was performed on 4th day of embryogenesis. Methionine at the dose of 50 mg/egg was dissolved in 250 ml of 0.7% NaCl and administered into the egg white (n = 25). Control group was treated with 250 ml of 0.7% NaCl (n = 25). Blood samples were taken from chicks on first day after hatch and 3 blood smears were made for each chick. Blood smears were stained with Hemacolor®. Leukocytograms were counted using 'two fields winding' method, and the results were averaged for each specimen. The data were analyzed statistically using the x2 test and the z test. In the control group, percentage of lymphocytes was 27.6, heterophils 43.9, eosinophils 19.3, basophils 3.3 and monocytes 5.9. Performed analyzes showed that methionine in ovo supplementation significantly changed picture of leukocytes in blood of one day-old chicks in comparison to control group ( p � 0.01). The main differences caused by methionine were an increase in the percentage of eosinophils to 30.4 ( p � 0.01), and a decrease in basophils and monocytes to 0.9 and 3.1% respectively ( p � 0.05). In summary, the obtained results indicate that a high dose of methionine administered in the early stage of embryogenesis affects the picture of leukocytes in the blood. Consequently, this may affect the formation of resistance, course of postembryonic development and production results. Therefore, it seems necessary to conduct further research.
Author K. B. Głodek (DoAS / DoPFABaAH)
K. B. Głodek,,
- Department of Poultry, Fur Animal Breeding and Animal Hygiene
, A. Ludwikowska (DoAS / DoPFABaAH)
A. Ludwikowska,,
- Department of Poultry, Fur Animal Breeding and Animal Hygiene
, Marcin Lis (DoAS / HBZ-KHDZFZ)
Marcin Lis,,
- Department of Poultry, Fur Animal Breeding and Animal Hygiene
, Barbara Tombarkiewicz (DoAS / HBZ-KHDZFZ)
Barbara Tombarkiewicz,,
- Department of Poultry, Fur Animal Breeding and Animal Hygiene
, Krzysztof Pawlak (DoAS / HBZ-KHDZFZ)
Krzysztof Pawlak,,
- Department of Poultry, Fur Animal Breeding and Animal Hygiene
, J. W. Niedziółka (DoAS / HBZ-KHDZFZ)
J. W. Niedziółka,,
- Department of Poultry, Fur Animal Breeding and Animal Hygiene
Journal seriesReproductive Biology, ISSN 1642-431X, (A 15 pkt)
Issue year2013
Vol13
No2
Pages36-37
Publication size in sheets0.3
ASJC Classification1309 Developmental Biology; 1310 Endocrinology; 1103 Animal Science and Zoology
DOIDOI:10.1016/j.repbio.2013.01.030
Languageen angielski
Score (nominal)15
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2013 = 0.63; WoS Impact Factor: 2013 = 1.048 (2) - 2013=1.479 (5)
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