Chondrogenic expression and DNA methylation patterns in prolonged passages of chondrocyte cell lines of the horse

T. Ząbek , W. Witarski , E. Semik-Gurgul , Tomasz Szmatoła , K. Kowalska , Monika Bugno-Poniewierska

Abstract

We investigated the activity of chondrogenic markers and variation of methylation patterns in equine cartilaginous cells cultivated in monolayer. The transcriptional and epigenetic effect of the long-term culture of chondrocytes has been evaluated using several passages of chondrocyte cell-lines derived from equine articular cartilage. Using 3 genes as endogenous control we tested the expression of 7 genes important for different stages of chondrocyte differentiation and maturation. CpG islands in RUNX3 locus were inspected for the evaluation of differential methylation state of passaged cell-lines. The general decline of transcript abundance of marker loci was detected in passage 11 which is the sign of dedifferentiation of cultivated chondrocytes in prolonged monolayer culture. Passages 13 and 14 were characterized by the upregulation of a number of genes, possibly due to the heterogeneity of developed cell lines at this stage of the culture. Instead, gradual increase of methylation percent at particular CpG sites of RUNX3 locus was associated with the growing number of passage. This finding led us to the conclusion that epigenetic alterations better describe the stage of cultivated chondrocytes.
Author T. Ząbek
T. Ząbek,,
-
, W. Witarski
W. Witarski,,
-
, E. Semik-Gurgul
E. Semik-Gurgul,,
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, Tomasz Szmatoła (VET)
Tomasz Szmatoła,,
- University Centre for Veterinary Medicine UJ-UR
, K. Kowalska
K. Kowalska,,
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, Monika Bugno-Poniewierska (FoAS / IoVS)
Monika Bugno-Poniewierska,,
- Institute of Veterinary Sciences
Journal seriesGene, ISSN 0378-1119, e-ISSN 1879-0038, (N/A 70 pkt)
Issue year2019
Vol707
Pages58-64
Publication size in sheets0.5
Keywords in EnglishArticular cartilage, Chondrocytes, Chondrogenic markers, DNA methylation, Horses
ASJC Classification2700 General Medicine; 1311 Genetics
DOIDOI:10.1016/j.gene.2019.05.018
URL https://www.sciencedirect.com/science/article/pii/S0378111919304809/pdfft?md5=369668c346ad5b552c5f3b47938004d7&pid=1-s2.0-S0378111919304809-main.pdf
Internal identifierWHiBZ/2019/19
Languageen angielski
Score (nominal)70
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.859; WoS Impact Factor: 2018 = 2.638 (2) - 2018=2.351 (5)
Citation count*
Additional fields
FinansowanieWork supported by the statutory project of the National Research Institute of Animal Production in Poland No. FBN 07-4.11.7.
OświadczenieT. Szmatoła - podwójna afiliacja
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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