Immunodetection, electrophoresis and amino acid composition of alcohol soluble proteins extracted from grains of selected varieties of pseudocereals, legumes, oat, maize and rice

Barbara Mickowska , Peter Socha , Dana Urminská , Ewa Cieślik

Abstract

Prolamins are alcohol-soluble fraction of cereal proteins involved in immunological re-sponse of patients with celiac disease. The aim of this study was to analyse the similar proteincomplex of selected varieties of cereal, pseudocereal andlegume grains by comparison of protein fractions, amino acids composition and SDS-PAGE electrophoresis. The immunoreactivity was tested by Western blot and ELISA methods. ELISA analysis recognizes celiac active epitopes in wheat gliadin (which is reference protein in determination of celiac activity),and also corresponding epitopes in other grain proteins responsible for immunological re-sponse of patients with celiac disease. Estimated quantity of celiac active sequences (calculated as gliadin content) below 20 ppm was found in species of amaranth, buckwheat and millet, as well as rice, maize, chickpea and chickling vetch. Immunological reaction with polyclonal antibody was negative for all crops, except oat, maize, millet and foxtail millet.
Author Barbara Mickowska (FoFT / DoToCaC / MCoFM)
Barbara Mickowska,,
- Małopolska Center of Food Monitoring
, Peter Socha
Peter Socha,,
-
, Dana Urminská
Dana Urminská,,
-
, Ewa Cieślik (FoFT / DoToCaC / MCoFM)
Ewa Cieślik,,
- Małopolska Center of Food Monitoring
Journal seriesCereal Research Communications, ISSN 0133-3720, (A 15 pkt)
Issue year2013
Vol41
No1
Pages160-169
Publication size in sheets0.5
Keywords in Englishprolamins, ELISA, Western blot, cereals, celiac disease
DOIDOI:10.1556/CRC.2012.0029
URL http://akademiai.com/doi/pdf/10.1556/CRC.2012.0029
Internal identifierWTŻ/2013/103
Languageen angielski
Score (nominal)15
ScoreMinisterial score = 15.0, 26-07-2017, ArticleFromJournal
Ministerial score (2013-2016) = 15.0, 26-07-2017, ArticleFromJournal
Citation count*
Additional fields
FinansowanieThis contribution is the result of the project implementation: Centre of excellence for white-green biotechnology, ITMS 26220120054, supported by the Research & Development Operational Programme funded by the ERDF.
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back
Confirmation
Are you sure?