Immunodetection, electrophoresis and amino acid composition of alcohol soluble proteins extracted from grains of selected varieties of pseudocereals, legumes, oat, maize and rice
Barbara Mickowska , Peter Socha , Dana Urminská , Ewa Cieślik
AbstractProlamins are alcohol-soluble fraction of cereal proteins involved in immunological re-sponse of patients with celiac disease. The aim of this study was to analyse the similar proteincomplex of selected varieties of cereal, pseudocereal andlegume grains by comparison of protein fractions, amino acids composition and SDS-PAGE electrophoresis. The immunoreactivity was tested by Western blot and ELISA methods. ELISA analysis recognizes celiac active epitopes in wheat gliadin (which is reference protein in determination of celiac activity),and also corresponding epitopes in other grain proteins responsible for immunological re-sponse of patients with celiac disease. Estimated quantity of celiac active sequences (calculated as gliadin content) below 20 ppm was found in species of amaranth, buckwheat and millet, as well as rice, maize, chickpea and chickling vetch. Immunological reaction with polyclonal antibody was negative for all crops, except oat, maize, millet and foxtail millet.
|Journal series||Cereal Research Communications, ISSN 0133-3720, (A 15 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||prolamins, ELISA, Western blot, cereals, celiac disease|
|Score|| = 15.0, 26-07-2017, ArticleFromJournal|
= 15.0, 26-07-2017, ArticleFromJournal
|Finansowanie||This contribution is the result of the project implementation: Centre of excellence for white-green biotechnology, ITMS 26220120054, supported by the Research & Development Operational Programme funded by the ERDF.|
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