Wpływ antagonisty glutaminianergicznego receptora metabotropowego mGluR1 na aktywność czepco-żwacza w teście doświadczalnego rozciągania ściany okrężnicy u owcy
Bogdan F. Kania , Małgorzata Wielgosz
AbstractThe aim of this study was defining relationships between a five minute episode of mechanical colonic distensions (CD) of different degrees, which were provoked by the insertion of a balloon filled with 150 and/or200 ml of water (CD150 and/or CD200), and reticulo-ruminal motility changes, heart rate, respiration frequency as vegetative symptoms of the nociceptive effects. Futhermore, noting the influence of premedication by metabotropic glutamate receptor antagonist DL-AP3 i.c.v. 1 min. infusion in 3 different doses (4.0, 8.0 and 12.0 mg in toto) on the above symptoms in sheep. The experiment was carried out in 4 series using 12 female sheep, Polish Merinos with fistula of dorsal sac of the rumen, in the anoestrus period. In the first series (6 animals as a control group) the animals had a 200 l 0.9% NaCl i.c.v. infusion. In the second series (6 animals) they had a 5 min episode of CD by insertion of a balloon filled with 150 and/or 200 ml of water (CD150 and/or CD200). In the third series of the experiment 6 animals had an i.c.v. DL-AP3 infusion in 3 different doses (4.0, 8.0 or 12.0 mg in toto), every week. In the fourth series, the animals had i.c.v. DL-AP3 infusions in 3 different doses (4.0, 8.0 or 12.0 mg in toto) ten minutes before CD. CD espisodes of five min duration provoke a very repetitive, statistically significant increase of heart rate/min, reticulo-ruminal motility inhibition, and nociceptive symptoms intensivity, and this can be a new model for testing potential analgesic substances and drugs for small ruminants. DL-AP3 racemate, a metabotropic glutamate receptor mGluR1 antagonist, can be an alternative drug in jejunal pain when it is provoked by colonic distension in sheep.
|Other language title versions||Influence of metabotropic glutamate receptor1 antagonist DL-AP3 on reticulo-ruminal motility and clinical/behavioral symptoms in experimental colonal distension in sheep|
|Journal series||Medycyna Weterynaryjna, ISSN 0025-8628, (A 15 pkt)|
|Keywords in English||sheep, colonic distension, DL-AP3, rumen motility, clinical/ behavioral symptoms|
|Publication indicators||: 2014 = 0.141; : 2013 = 0.196 (2) - 2015=0.162 (5)|
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