Structural, rheological and functional properties of galactose-rich pectic polysaccharide fraction from leek

Manol Ognyanov , Connie Remoroza , Henk A. Schols , Yordan Georgiev , Nadezhda Petkova , Magdalena Krystyjan

Abstract

An acid-extracted polysaccharide from alchohol-insoluble solids of leek was obtained. The sugar composition indicated that galactose and galacturonic acid were the major sugars, followed by small amounts of rhamnose and arabinose. The fraction contained a relatively high methyl-esterified homogalacturonan next to rhamnogalacturonan type I decorated with galactose-rich side chains. The fraction consisted of three high Mw populations, covering the range of 10–100 kDa. Enzymatic fingerprinting was performed with HG/RG-I degrading enzymes to elucidate the structure. The oligomers were analysed using LC-HILIC-MS, HPAEC, and MALDI-TOF MS. The data revealed the presence of GalA sequences, having different patterns of methyl-esterification, RG-I composed of unbranched segments and segments heavily substituted with β-(1→4)-linked galactan chains of varying length. The rheological study showed the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior of the polysaccharide. The pectin exhibited higher water holding capacity than oil-holding capacity and the fraction did form stable foams at high concentration.
Author Manol Ognyanov
Manol Ognyanov,,
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, Connie Remoroza
Connie Remoroza,,
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, Henk A. Schols
Henk A. Schols,,
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, Yordan Georgiev
Yordan Georgiev,,
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, Nadezhda Petkova
Nadezhda Petkova,,
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, Magdalena Krystyjan (FoFT / DoCT)
Magdalena Krystyjan,,
- Department of Carbohydrate Technology
Journal seriesCarbohydrate Polymers, ISSN 0144-8617, e-ISSN 1879-1344, (N/A 140 pkt)
Issue year2020
Vol229
Pages1-11
Publication size in sheets0.5
Article number115549
Keywords in EnglishLeek, Pectic polysaccharides, Enzymatic fingerprinting, HILIC-MS, Rheology
ASJC Classification1605 Organic Chemistry; 2505 Materials Chemistry; 2507 Polymers and Plastics
DOIDOI:10.1016/j.carbpol.2019.115549
URL https://www.sciencedirect.com/science/article/pii/S0144861719312172/pdfft?md5=7474f373301e8377640bee46488d7817&pid=1-s2.0-S0144861719312172-main.pdf
Languageen angielski
Score (nominal)140
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.72; WoS Impact Factor: 2018 = 6.044 (2) - 2018=5.975 (5)
Citation count*
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FinansowanieThis study was supported by Project BG051PO001/3.3-05 “Science and Business”, financed by the European Social Fund of the European Union and Bulgarian Ministry of Education and Science and partly by Project BG161PO003-1.1.05-0024-C0001 “Development of nutraceuticals with antioxidant and immune-stimulating action” of Operational Program “Competitiveness” of the European Union.
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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