Identification of Antioxidant Peptides in Enzymatic Hydrolysates of Carp (Cyprinus Carpio) Skin Gelatin

Joanna Tkaczewska , Michal Bukowski , Paweł Mak

Abstract

The protein by-products from carp (Cyprinus carpio) are normally discarded as industrial waste during fish processing. The objective of this study was to identify and characterise the peptides with a potential antioxidant activity that are released from carp skin proteins during hydrolysis by the Protamex enzyme mixture. This study shows that a hydrolysate of carp skin gelatin and its reversed-phase chromatography fractions have strong in vitro antioxidant properties. Among these fractions, the alanine-tyrosine (Ala-Tyr) dipeptide was identified as the major compound with high antioxidant potential. The peptide has good stability during in vitro enzymatic digestion assay and can inhibit the angiotensin-converting enzyme (ACE). In conclusion, our study proves that both the unfractionated hydrolysate of carp skin gelatin and the above-mentioned Ala-Tyr dipeptide represents attractive novel compounds for the formulation of antioxidant foods.
Author Joanna Tkaczewska (FoFT / DoAPT)
Joanna Tkaczewska,,
- Department of Animal Product Technology
, Michal Bukowski
Michal Bukowski,,
-
, Paweł Mak
Paweł Mak,,
-
Journal seriesMolecules, ISSN 1420-3049, (N/A 100 pkt)
Issue year2019
Vol24
No1
Pages1-15
Publication size in sheets0.7
Keywords in Englishgelatin; bioactive peptides; fish skin; antioxidant; Ala-Tyr; identification
ASJC Classification1605 Organic Chemistry
DOIDOI:10.3390/molecules24010097
URL https://www.mdpi.com/1420-3049/24/1/97/pdf
Internal identifierWTŻ/2019/9
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
Score (nominal)100
Score sourcejournalList
Publication indicators WoS Citations = 2; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.146; WoS Impact Factor: 2018 = 3.06 (2) - 2018=3.38 (5)
Citation count*
Additional fields
FinansowanieThis work was supported by the National Centre for Research and Development, Poland [Grant no: Lider/21/0003/l-7/15/NCBR/2016]. The Faculty of Biochemistry, Biophysics and Biotechnology of Jagiellonian University in Krakow is a partner of the Leading National Research Center (KNOW) supported by the Ministry of Science and Higher Education (Warszawa, Poland).
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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