Identification of Antioxidant Peptides in Enzymatic Hydrolysates of Carp (Cyprinus Carpio) Skin Gelatin
Joanna Tkaczewska , Michal Bukowski , Paweł Mak
AbstractThe protein by-products from carp (Cyprinus carpio) are normally discarded as industrial waste during fish processing. The objective of this study was to identify and characterise the peptides with a potential antioxidant activity that are released from carp skin proteins during hydrolysis by the Protamex enzyme mixture. This study shows that a hydrolysate of carp skin gelatin and its reversed-phase chromatography fractions have strong in vitro antioxidant properties. Among these fractions, the alanine-tyrosine (Ala-Tyr) dipeptide was identified as the major compound with high antioxidant potential. The peptide has good stability during in vitro enzymatic digestion assay and can inhibit the angiotensin-converting enzyme (ACE). In conclusion, our study proves that both the unfractionated hydrolysate of carp skin gelatin and the above-mentioned Ala-Tyr dipeptide represents attractive novel compounds for the formulation of antioxidant foods.
|Journal series||Molecules, ISSN 1420-3049, (N/A 100 pkt)|
|Publication size in sheets||0.7|
|Keywords in English||gelatin; bioactive peptides; fish skin; antioxidant; Ala-Tyr; identification|
|License||Journal (articles only); author's original; ; after publication|
|Publication indicators||= 2; : 2017 = 1.146; : 2018 = 3.06 (2) - 2018=3.38 (5)|
|Finansowanie||This work was supported by the National Centre for Research and Development, Poland [Grant no: Lider/21/0003/l-7/15/NCBR/2016]. The Faculty of Biochemistry, Biophysics and Biotechnology of Jagiellonian University in Krakow is a partner of the Leading National Research Center (KNOW) supported by the Ministry of Science and Higher Education (Warszawa, Poland).|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.