In vitro effects of PNP and PNMC on apoptosis and proliferation in the hen ovarian stroma and prehierarchal follicles

Agnieszka Grzegorzewska , Anna Hrabia , Kinga Kowalik , Dorota Katarzyńska-Banasik , Anna Kozubek , Andrzej Sechman

Abstract

This study aimed to examine the mRNA expression, activity, and immunolocalisation of apoptosis/proliferation regulating factors following in vitro exposure of the stroma, white (WFs), and yellowish (YFs) follicles of the chicken ovary to 4-nitrophenol (PNP) or 3-methyl-4-nitrophenol (PNMC). PNMC increased the mRNA expression of caspase-3, -8, Apaf-1, and cytochrome c in the ovarian stroma. The activity of caspase-3, -8, and -9 decreased in WFs in both nitrophenol-treated groups. PNP reduced the number of caspase-3-positive cells in the stromal connective tissue (CT) and the theca interna and externa layers of WFs. In the stroma, the proliferating index decreased in the wall of primary follicles in both nitrophenol-treated groups, however, in the CT, the effect of PNMC was opposite. In the theca interna of WFs, PNP diminished the proliferating index. These results suggest that nitrophenols might impact the development of chicken ovarian follicles by affecting cell death and proliferation.
Author Agnieszka Grzegorzewska (DoAS / DoAPaE)
Agnieszka Grzegorzewska,,
- Department of Animal Physiology and Endocrinology
, Anna Hrabia (FoAS / DoAPaE)
Anna Hrabia,,
- Department of Animal Physiology and Endocrinology
, Kinga Kowalik (DoAS / DoAPaE)
Kinga Kowalik,,
- Department of Animal Physiology and Endocrinology
, Dorota Katarzyńska-Banasik (FoAS / DoAPaE)
Dorota Katarzyńska-Banasik,,
- Department of Animal Physiology and Endocrinology
, Anna Kozubek (FoAS / DoAPaE)
Anna Kozubek,,
- Department of Animal Physiology and Endocrinology
, Andrzej Sechman (FoAS / DoAPaE)
Andrzej Sechman,,
- Department of Animal Physiology and Endocrinology
Journal seriesActa Histochemica, ISSN 0065-1281, e-ISSN 1618-0372, (N/A 70 pkt)
Issue year2020
Vol122
No1
Pages1-12
Publication size in sheets0.55
Article number151463
Keywords in EnglishPNP, PNMC, Apoptosis, Proliferation, Ovary, Chicken
ASJC Classification2700 General Medicine; 2722 Histology; 1307 Cell Biology
DOIDOI:10.1016/j.acthis.2019.151463
URL https://www.sciencedirect.com/science/article/pii/S0065128119303083/pdfft?md5=65ca5916fbf70a29e1c75bba5b1a6a6b&pid=1-s2.0-S0065128119303083-main.pdf
Languageen angielski
Score (nominal)70
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.668; WoS Impact Factor: 2018 = 1.719 (2) - 2018=1.594 (5)
Citation count*
Additional fields
FinansowanieThis work was financially supported by the National Science Center, Poland (grant no. UMO-2014/15/B/NZ9/01986) dedicated to A. S.
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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