In vitro effects of PNP and PNMC on apoptosis and proliferation in the hen ovarian stroma and prehierarchal follicles
Agnieszka Grzegorzewska , Anna Hrabia , Kinga Kowalik , Dorota Katarzyńska-Banasik , Anna Kozubek , Andrzej Sechman
AbstractThis study aimed to examine the mRNA expression, activity, and immunolocalisation of apoptosis/proliferation regulating factors following in vitro exposure of the stroma, white (WFs), and yellowish (YFs) follicles of the chicken ovary to 4-nitrophenol (PNP) or 3-methyl-4-nitrophenol (PNMC). PNMC increased the mRNA expression of caspase-3, -8, Apaf-1, and cytochrome c in the ovarian stroma. The activity of caspase-3, -8, and -9 decreased in WFs in both nitrophenol-treated groups. PNP reduced the number of caspase-3-positive cells in the stromal connective tissue (CT) and the theca interna and externa layers of WFs. In the stroma, the proliferating index decreased in the wall of primary follicles in both nitrophenol-treated groups, however, in the CT, the effect of PNMC was opposite. In the theca interna of WFs, PNP diminished the proliferating index. These results suggest that nitrophenols might impact the development of chicken ovarian follicles by affecting cell death and proliferation.
|Journal series||Acta Histochemica, ISSN 0065-1281, e-ISSN 1618-0372, (N/A 70 pkt)|
|Publication size in sheets||0.55|
|Keywords in English||PNP, PNMC, Apoptosis, Proliferation, Ovary, Chicken|
|ASJC Classification||; ;|
|Publication indicators||= 0; : 2018 = 0.668; : 2018 = 1.719 (2) - 2018=1.594 (5)|
|Finansowanie||This work was financially supported by the National Science Center, Poland (grant no. UMO-2014/15/B/NZ9/01986) dedicated to A. S.|
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