HSP Transcript and Protein Accumulation in Brassinosteroid Barley Mutants Acclimated to Low and High Temperatures

Iwona Sadura , Maria Libik-Konieczny , Barbara Jurczyk , Damian Gruszka , Anna Janeczko

Abstract

In temperature stress, the main role of heat-shock proteins (HSP) is to act as molecular chaperones for other cellular proteins. However, knowledge about the hormonal regulation of the production of the HSP is quite limited. Specifically, little is known about the role of the plant steroid hormones—brassinosteroids (BR)—in regulating the HSP expression. The aim of our study was to answer the question of how a BR deficit or disturbances in its signaling affect the accumulation of the HSP90, HSP70, HSP18, and HSP17 transcripts and protein in barley growing at 20 °C (control) and during the acclimation of plants at 5 °C and 27 °C. In barley, the temperature of plant growth modified the expression of HSPs. Furthermore, the BR-deficient mutants (mutations in the HvDWARF or HvCPD genes) and BR-signaling mutants (mutation in the HvBRI1 gene) were characterized by altered levels of the transcripts and proteins of the HSP group compared to the wild type. The BR-signaling mutant was characterized by a decreased level of the HSP transcripts and heat-shock proteins. In the BR-deficient mutants, there were temperature-dependent cases when the decreased accumulation of the HSP70 and HSP90 transcripts was connected to an increased accumulation of these HSP. The significance of changes in the accumulation of HSPs during acclimation at 27 °C and 5 °C is discussed in the context of the altered tolerance to more extreme temperatures of the studied mutants (i.e., heat stress and frost, respectively).
Author Iwona Sadura - Instytut Fizjologii Roślin im. Franciszka Górskiego Polskiej Akademii Nauk
Iwona Sadura,,
-
, Maria Libik-Konieczny - Instytut Fizjologii Roślin im. Franciszka Górskiego Polskiej Akademii Nauk
Maria Libik-Konieczny,,
-
, Barbara Jurczyk (FoAaE / DoPoPBaSP)
Barbara Jurczyk,,
- Department of Physiology of Plant Breeding and Seed Production
, Damian Gruszka - Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia
Damian Gruszka,,
-
, Anna Janeczko - Instytut Fizjologii Roślin im. Franciszka Górskiego Polskiej Akademii Nauk
Anna Janeczko,,
-
Journal seriesInternational Journal of Molecular Sciences, ISSN 1422-0067, (N/A 140 pkt)
Issue year2020
Vol21
No5
Pages1-18
Publication size in sheets0.85
Article number1889
Keywords in Englishbrassinosteroids; acclimation process; small HSPs (sHSPs); HSP70; HSP90; temperature stress
ASJC Classification2700 General Medicine; 1312 Molecular Biology; 1503 Catalysis; 1604 Inorganic Chemistry; 1605 Organic Chemistry; 1606 Physical and Theoretical Chemistry; 1607 Spectroscopy; 1706 Computer Science Applications
DOIDOI:10.3390/ijms21051889
URL https://www.mdpi.com/1422-0067/21/5/1889/pdf
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
File
HSP Transcript and Protein Accumulation in Brassinosteroid Barley Mutants Acclimated to Low and High Temperatures of 08-05-2020
3,9 MB
Score (nominal)140
Score sourcejournalList
Publication indicators WoS Citations = 1; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.224; WoS Impact Factor: 2018 = 4.183 (2) - 2018=4.331 (5)
Citation count*
Additional fields
Finansowanie The work was funded by the Polish National Science Center grant number 2015/17/B/NZ9/01695.
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back
Confirmation
Are you sure?