Effect of Aroclor 1254 on LH and 17,20ß - P secretion in female Prussian carp (Carassius gibelio Bloch) in the spawning season

Magdalena Socha , Mirosława Sokołowska-Mikołajczyk , Paweł Szczerbik , Jarosław Chyb , Piotr Epler

Abstract

The effects of Aroclor 1254 on the secretion of luteinizing hormone (LH) and 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) in female Prussian carp (Carassius gibelio Bloch) at the time of their natural spawning were determined. Aroclor 1254 at doses of 0.01, 0.1, and 1 mg/kg body weight was dissolved in 500 ml of oil and was administered three times intraperitoneally or rectally to female Prussian carp every 24 h for three days. Blood samples were collected after 3 days of Aroclor 1254 administration to determine the LH and 17,20β-P concentrations using ELISA. At 6, 12, and 24 h after injection of gonadotropin-releasing hormone analogue (GnRH-A) blood samples were collected for stimulated LH secretion determination. Aroclor 1254 administered intraperitoneally (0.1 and 1 mg/kg) and rectally (0.01 and 1 mg/kg) significantly increased spontaneous LH secretion. In the case of GnRH-A-stimulated LH release, Aroclor 1254 (administered intraperitoneally only) at concentrations of 0.1 and 1 mg/kg significantly decreased gonadotropin release. The intraperitoneal injections of the lowest tested concentration of Aroclor 1254 also significantly decreased 17,20β-P secretion. The results show that Aroclor 1254 can affect the reproductive system of Prussian carp by changing the secretion of two very important hormones, LH and 17,20β-P, at the time of natural spawning.
Author Magdalena Socha (DoAS / DoIaF)
Magdalena Socha,,
- Department of Ichthyobiology and Fisheries
, Mirosława Sokołowska-Mikołajczyk (DoAS / DoIaF)
Mirosława Sokołowska-Mikołajczyk,,
- Department of Ichthyobiology and Fisheries
, Paweł Szczerbik (DoAS / DoIaF)
Paweł Szczerbik,,
- Department of Ichthyobiology and Fisheries
, Jarosław Chyb (DoAS / DoIaF)
Jarosław Chyb,,
- Department of Ichthyobiology and Fisheries
, Piotr Epler (DoAS / DoIaF)
Piotr Epler,,
- Department of Ichthyobiology and Fisheries
Journal seriesCzech Journal of Animal Science, ISSN 1212-1819, (A 30 pkt)
Issue year2013
Vol58
No8
Pages375-380
Publication size in sheets0.5
Keywords in EnglishPCBs, cyprinids, gonadotropins, LH, steroids, reproduction
ASJC Classification1103 Animal Science and Zoology
URL http://www.agriculturejournals.cz/publicFiles/97598.pdf
Internal identifierWHiBZ/2013/84
Languageen angielski
Score (nominal)30
Score sourcejournalList
Publication indicators WoS Citations = 1; Scopus SNIP (Source Normalised Impact per Paper): 2013 = 0.87; WoS Impact Factor: 2013 = 0.871 (2) - 2013=0.857 (5)
Citation count*
Additional fields
FinansowanieSupported by the Ministry of Science and Higher Education, Poland (Grant No. N311 032 32/2322)
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