The effect of docosahexaenoic acid-rich algae supplementation in milk replacer on performance and selected immune system functions in calves

Jadwiga Flaga , Łukasz Korytkowski , Paweł Górka , Zygmunt Kowalski

Abstract

The aim of this study was to determine the effect of docosahexaenoic acid-rich algae (DHA-RA) supplementation in milk replacer (MR) on performance, selected cytokine expression in lymphocytes, and blood immunoglobulin concentration in newborn dairy calves. Forty female Holstein-Friesian calves (8.6 ± 0.8 d old and 41.1 ± 4.3 kg; mean ± standard deviation) were blocked by date of birth and allocated into 4 experimental groups (10 animals/group): (1) not supplemented with DHA-RA, (2) supplemented with 9 g of DHA-RA/d in MR, (3) supplemented with 18 g of DHA-RA/d in MR, and (4) supplemented with 27 g of DHA-RA/d in MR. Milk replacer was fed in an amount equal to 900 g of MR powder/d (as fed), 2 times a d, for 49 d. Starter mixture (SM) was fed ad libitum beginning on d 15 of the study. Each calf was in the study over a period of 49 d. The MR and SM intake and fecal score were recorded daily and body weight was recorded weekly. Blood samples were collected before the morning feeding, at the beginning of the study, every consecutive week, and at the end of the study for morphology and smear analysis, serum immunoglobulin level (IgG, IgA, and IgM), and lymphocyte isolation. The mRNA isolated from lymphocytes was checked for TNFα, IL-1β, IL-6, and p65 expression. Average daily gain between d 1 to 14 of the study increased quadratically with increasing dose of DHA-RA. However, average daily gain between d 15 to 49 of the study tended to linearly decrease and over the whole study linearly decreased with increasing dose of DHA-RA. The MR intake decreased linearly between d 1 to 14 of the study and over the whole study, and mean SM intake decreased quadratically with increasing dose of DHA-RA. Feed efficiency increased quadratically and fecal score decreased quadratically during the first 14 d of the study. Increasing dose of DHA-RA led to cubic changes in feed efficiency and fecal score between d 15 and 49 of the study. Overall, over the whole study period a tendency was observed for lower fecal score for the DHA-RA supplemented groups. Interleukin-1β mRNA expression decreased linearly, whereas the mRNA expression of p65 and TNFα as well as serum IgG concentration tended to decrease linearly with increasing dose of supplemental DHA-RA. No effect of group was found on IgA and IgM serum level and the majority of blood parameters. Altogether, treatment worsened production variables but seemed to have a beneficial effect on the immune system of calves.
Author Jadwiga Flaga (FoAS / DoANaD)
Jadwiga Flaga,,
- Department of Animal Nutrition and Dietetics
, Łukasz Korytkowski (FoAS / DoANaD)
Łukasz Korytkowski,,
- Department of Animal Nutrition and Dietetics
, Paweł Górka (FoAS / DoANaD)
Paweł Górka,,
- Department of Animal Nutrition and Dietetics
, Zygmunt Kowalski (FoAS / DoANaD)
Zygmunt Kowalski,,
- Department of Animal Nutrition and Dietetics
Journal seriesJournal of Dairy Science, ISSN 0022-0302, e-ISSN 1525-3198, (N/A 200 pkt)
Issue year2019
Vol102
No10
Pages8862-8873
Publication size in sheets0.55
Keywords in Englishdocosahexaenoic acid, immunoglobulin, calf, inflammatory cytokine
ASJC Classification1103 Animal Science and Zoology; 1106 Food Science; 1311 Genetics
DOIDOI:10.3168/jds.2018-16189
URL https://reader.elsevier.com/reader/sd/pii/S0022030219306976?token=5EF7D95E526B6F028575337E7D312CB972E6D934218995206D27CFD0B8489D8A96A24F4DFBB7B28269D663F8E451CBE6
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa - Użycie Niekomercyjne - Bez utworów zależnych (CC-BY-NC-ND); after publication
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The effect of docosahexaenoic acid-rich algae supplementation in milk replacer on performance and selected immune system functions in calves of 27-09-2019
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Score (nominal)200
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.557; WoS Impact Factor: 2018 = 3.082 (2) - 2018=3.208 (5)
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FinansowanieThis investigation was supported by the Ministry of Science and Higher Education in Poland (DS-3217/KŻiDZ/2016 and BM-4253/KŻiDZ/2016). The authors thank graduate students of the Department of Animal Nutrition and Dietetics, University of Agriculture in Krakow, Michał Miłobędzki and Katarzyna Pawlik, for their technical assistance
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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