The effect of docosahexaenoic acid-rich algae supplementation in milk replacer on performance and selected immune system functions in calves

Jadwiga Flaga , Łukasz Korytkowski , Paweł Górka , Zygmunt Kowalski

Abstract

The aim of this study was to determine the effect of docosahexaenoic acid-rich algae (DHA-RA) supplementation in milk replacer (MR) on performance, selected cytokine expression in lymphocytes, and blood immunoglobulin concentration in newborn dairy calves. Forty female Holstein-Friesian calves (8.6 ± 0.8 d old and 41.1 ± 4.3 kg; mean ± standard deviation) were blocked by date of birth and allocated into 4 experimental groups (10 animals/group): (1) not supplemented with DHA-RA, (2) supplemented with 9 g of DHA-RA/d in MR, (3) supplemented with 18 g of DHA-RA/d in MR, and (4) supplemented with 27 g of DHA-RA/d in MR. Milk replacer was fed in an amount equal to 900 g of MR powder/d (as fed), 2 times a d, for 49 d. Starter mixture (SM) was fed ad libitum beginning on d 15 of the study. Each calf was in the study over a period of 49 d. The MR and SM intake and fecal score were recorded daily and body weight was recorded weekly. Blood samples were collected before the morning feeding, at the beginning of the study, every consecutive week, and at the end of the study for morphology and smear analysis, serum immunoglobulin level (IgG, IgA, and IgM), and lymphocyte isolation. The mRNA isolated from lymphocytes was checked for TNFα, IL-1β, IL-6, and p65 expression. Average daily gain between d 1 to 14 of the study increased quadratically with increasing dose of DHA-RA. However, average daily gain between d 15 to 49 of the study tended to linearly decrease and over the whole study linearly decreased with increasing dose of DHA-RA. The MR intake decreased linearly between d 1 to 14 of the study and over the whole study, and mean SM intake decreased quadratically with increasing dose of DHA-RA. Feed efficiency increased quadratically and fecal score decreased quadratically during the first 14 d of the study. Increasing dose of DHA-RA led to cubic changes in feed efficiency and fecal score between d 15 and 49 of the study. Overall, over the whole study period a tendency was observed for lower fecal score for the DHA-RA supplemented groups. Interleukin-1β mRNA expression decreased linearly, whereas the mRNA expression of p65 and TNFα as well as serum IgG concentration tended to decrease linearly with increasing dose of supplemental DHA-RA. No effect of group was found on IgA and IgM serum level and the majority of blood parameters. Altogether, treatment worsened production variables but seemed to have a beneficial effect on the immune system of calves.
Autor Jadwiga Flaga (HBZ / HBZ-KŻDZ)
Jadwiga Flaga
- Katedra Żywienia i Dietetyki Zwierząt
, Łukasz Korytkowski (HBZ / HBZ-KŻDZ)
Łukasz Korytkowski
- Katedra Żywienia i Dietetyki Zwierząt
, Paweł Górka (HBZ / HBZ-KŻDZ)
Paweł Górka
- Katedra Żywienia i Dietetyki Zwierząt
, Zygmunt Kowalski (HBZ / HBZ-KŻDZ)
Zygmunt Kowalski
- Katedra Żywienia i Dietetyki Zwierząt
Tytuł czasopisma/seriiJournal of Dairy Science, ISSN 0022-0302, e-ISSN 1525-3198, (N/A 200 pkt)
Rok wydania2019
Tom102
Nr10
Paginacja8862-8873
Objętość publikacji w arkuszach wydawniczych0.55
Słowa kluczowe w języku angielskimdocosahexaenoic acid, immunoglobulin, calf, inflammatory cytokine
Klasyfikacja ASJC1103 Animal Science and Zoology; 1106 Food Science; 1311 Genetics
DOIDOI:10.3168/jds.2018-16189
URL https://reader.elsevier.com/reader/sd/pii/S0022030219306976?token=5EF7D95E526B6F028575337E7D312CB972E6D934218995206D27CFD0B8489D8A96A24F4DFBB7B28269D663F8E451CBE6
Języken angielski
LicencjaCzasopismo (tylko dla artykułów); autorska oryginalna; Uznanie Autorstwa - Użycie Niekomercyjne - Bez utworów zależnych (CC-BY-NC-ND); po opublikowaniu
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The effect of docosahexaenoic acid-rich algae supplementation in milk replacer on performance and selected immune system functions in calves z dnia 27-09-2019
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Punktacja (całkowita)200
Żródło punktacjijournalList
Wskaźniki publikacji Cytowania WoS = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.557; Impact Factor WoS: 2018 = 3.082 (2) - 2018=3.208 (5)
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FinansowanieThis investigation was supported by the Ministry of Science and Higher Education in Poland (DS-3217/KŻiDZ/2016 and BM-4253/KŻiDZ/2016). The authors thank graduate students of the Department of Animal Nutrition and Dietetics, University of Agriculture in Krakow, Michał Miłobędzki and Katarzyna Pawlik, for their technical assistance
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* Podana liczba cytowań wynika z analizy informacji dostępnych w Internecie i jest zbliżona do wartości obliczanej przy pomocy systemu Publish or Perish.
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