Expression of gelatinases (MMP-2 and MMP-9) and tissue inhibitors of metalloproteinases (TIMP-2 and TIMP-3) in the chicken ovary in relation to follicle development and atresia

Anna Hrabia , Dominika Wolak , Maria Kwaśniewska , Anna Kierońska , Joanna Socha , Andrzej Sechman

Abstract

Matrix metalloproteinases (MMPs) are a family of peptidases that possess the ability to break down extracellular matrix macromolecules associated with tissue turnover in various physiological and pathological conditions. Their activity is largely regulated by specific tissue inhibitors of MMPs (TIMPs). Information concerning the role of MMPs in the chicken ovary is very limited. The aim of the present study was to determine the expression and localization of selected members of the MMP system in different compartments of the laying hen ovary and to investigate whether their expression changes at different stages of the ovulatory cycle. MMP-2 and -9 activity was also examined. Expression of MMP-2, -9 and tissue inhibitors of MMPs (TIMP-2 and -3) in the ovarian follicles was examined 22 h and 3 h before F1 ovulation. Real-time polymerase chain reaction and western blot revealed differential mRNA and protein expression of MMP-2, MMP-9, TIMP-2, and TIMP-3 in the ovarian follicles: white, yellowish, small yellow, the largest preovulatory (F3-F1), and white atretic. Within the ovary, the relative expression of MMP and TIMP mRNA depended on follicle development, the layer of follicular wall, and ovulation stage. The relatively higher expression of MMP-2 and MMP-9 mRNA in the ovarian follicles 3 h compared to 22 h before ovulation was found. As follicle development progressed toward ovulation, elevated MMP-2 and -9 activity was noted. Atresia of white follicles was accompanied by an increase in gelatinase activities. Immunohistochemistry demonstrated tissue- and follicle-dependent immunoreactivity of the examined MMPs and TIMPs. In summary, the results show tissue- and stage of the ovulatory cycle-dependent differences in MMP and TIMP expression, as well as MMP-2 and -9 activity. Findings that suggest these molecules might significantly participate in the complex remodeling of extracellular matrix required for follicle development, ovulation, and atresia in the chicken ovary.
Author Anna Hrabia (FoAS / DoAPaE)
Anna Hrabia,,
- Department of Animal Physiology and Endocrinology
, Dominika Wolak (FoAS / DoAPaE)
Dominika Wolak,,
- Department of Animal Physiology and Endocrinology
, Maria Kwaśniewska (DoAS / DoAPaE)
Maria Kwaśniewska,,
- Department of Animal Physiology and Endocrinology
, Anna Kierońska (FoAS / DoAPaE)
Anna Kierońska,,
- Department of Animal Physiology and Endocrinology
, Joanna Socha (FoAS / DoAPaE)
Joanna Socha,,
- Department of Animal Physiology and Endocrinology
, Andrzej Sechman (DoAS / DoAPaE)
Andrzej Sechman,,
- Department of Animal Physiology and Endocrinology
Journal seriesTheriogenology, ISSN 0093-691X, e-ISSN 1879-3231, (N/A 140 pkt)
Issue year2019
Vol125
Pages268-276
Publication size in sheets0.5
Keywords in EnglishMMPs, TIMPs, Remodeling, Ovary, Chicken
ASJC Classification3402 Equine; 3403 Food Animals; 3404 Small Animals; 1103 Animal Science and Zoology
DOIDOI:10.1016/j.theriogenology.2018.11.017
URL https://pdf.sciencedirectassets.com/271179/1-s2.0-S0093691X18X00181/1-s2.0-S0093691X18306836/main.pdf?x-amz-security-token=AgoJb3JpZ2luX2VjEMD%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEaCXVzLWVhc3QtMSJIMEYCIQC3TNMhGcDG8qFinMJyFg6Z6EafdncBtuL3gkqNXbFgpwIhAMrmka2YviLAD8t4lTFZ0cH5GJXVjYedT%2FW%2BA4AIPUNaKuMDCLj%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEQAhoMMDU5MDAzNTQ2ODY1IgxEPTdTtUJKy1xDb%2FkqtwNtF2ID7CcfTzGQr0RFfC6IsVLJVMNxNVL%2F%2FsfpXkH%2F5iGjFdHAOHCkn0FMQmrwDwmt0gXtnb4CK3NRQX2D19LjVRyRL0TfPU0zD7KB1sVCgP4xQzD7PkInDqKFlsxdSqkFUj33GfKUIg7Ce0%2BQsjvsBTuJFUKvqS2icGy1uLPGi0czWjbR1x%2FVuBrEJwgOBZCfamQRmbhiQvnXuxLf%2Ft6KvD4k6UcQ4S%2BpCTWfNdxs4HnhP94EDndUB2Z6IdNy9k0hNxsSU4oOFCt1ZABJur1wJ%2Fb6%2FXgGeZ9335Gtv%2BvvILfQ9%2FoJSsTCrGRyiV%2F2hw8WL%2BdLslQJXXBEdTY9%2BcoRxHxLbFRzIRrpyih%2Bc8SA40TEfPhPU2%2BzmUOcyirdEF6jkqCTuqDxJBsU%2FfVzQfIMwXKhu3vu3yHzrWvEd0zjnm31xW7e9JA0yuh6f%2B0l%2F%2FL9zLEr2ee10JgEYoD2WwCg29skG11YH0zb8U%2BiFBwFK7jKipd8zQhMLD6IdbfP%2FBvQoNMI4DY29gMILUogF%2BXgljD2paFAoRT7Zxttx6ky8dFGBFnO7fuAb8OJOLDHSKCypcVhFAwkMPGv5OYFOrMB7iGsEZ6ePiRnK80FJH%2BChs4TcwnzN9pXeJDPGQ8vvZeU0ZNXEgj6jbtbJ%2BmtTPomszR5ptChmCbffztJqLm6ygI9Ir5OAHTM8Me%2BqXIdvZ73OjD9Ct0SyEOTD1DA634XDw6dZlpC%2BCWFvyhak32OI23IegM2d5FH09LpRLa%2FUTe4X6uB%2FRHHzG0olPcVvSrnviiRrJ58DyMC4XiIENN8OBaRZp8eJvHwYpzBxjilSfb3GZY%3D&AWSAccessKeyId=ASIAQ3PHCVTY72TFLTV3&Expires=1557734979&Signature=N6WgQtjOYKwJfq4D%2FVTVrexHMd4%3D&hash=26f54db5dca6c1819fd152f1dce2eb55dbfc7b1428e5574a764df147d4ee1f8a&host=68042c943591013ac2b2430a89b270f6af2c76d8dfd086a07176afe7c76c2c61&pii=S0093691X18306836&tid=spdf-bb128325-4d75-4e1a-8385-9f0133eb4e5d&sid=fab577a26748724b425ac5652f698837e493gxrqb&type=client
Internal identifierWHiBZ/2019/18
Languageen angielski
Score (nominal)140
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.338; WoS Impact Factor: 2018 = 2.299 (2) - 2018=2.316 (5)
Citation count*
Additional fields
FinansowanieThis work was financially supported by the National Science Center, Poland (Grant OPUS No. UMO-2015/19/B/NZ9/01356 dedicated to A.H.)
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