Possible sensor applications of selected DNA–surfactant complexes
Ewelina Nowak , Anna Wisła-Świder , Gohar Khachatryan , Maciej Fiedorowicz , Krzysztof Danel
AbstractAlthough much research has been performed on DNA complexes carrying long alkyl chains (C10, C16, and C18), there is no information about physicochemical characterization of synthesized composites with allyl imidazole-based ionic liquids and quaternary ammonium salts with n-butyl chains. Here, complexes were synthesized by ion-exchange reactions between sonicated DNA and three ionic liquids (ILs) formed from two imidazole-based compounds, 1-allyl-3-methylimidazolium bromide (Amim) or 1-butyl-3-methylimidazolium bromide (Bmim), and from the quaternary ammonium salt tetra-n-butylammonium bromide (TBAB). Signals in UV–Vis, IR, and CD spectra indicating inclusion of small molecules into the DNA structure confirmed the formation of DNA complexes. Both IR and CD spectra indicated that the B-form conformation of the DNA did not change after the formation of the complexes. Similarly, X-ray diffraction patterns revealed that the formation of IL–DNA complexes did not change the structure of native B-form DNA. Molecular weight (Mw) and radii of gyration (Rg) values of IL–DNA complex chains, established by high-performance size exclusion chromatography coupled with multiangle-laser light-scattering with a differential refractive index detector, were significantly lower than those values found for native DNA molecules due to DNA fragmentation by sonication during complex formation and the direct effects of the IL on the DNA. Scanning electron microscopy images indicate the formation of nanofibres in DNA-Amim and DNA-Bmim complexes, whereas the formation of nanowires was found in samples of DNA-TBAB complexes. Changes in optical properties confirmed by UV and photoluminescence make DNA–IL complexes potential candidates for biosensor application.
|Journal series||European Biophysics Journal With Biophysics Letters, [European Biophysics Journal], ISSN 0175-7571, e-ISSN 1432-1017, (N/A 70 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Deoxyribonucleic acids, Biopolymers, Imidazoles, Ionic liquids, Ion-exchange reactions|
|License||Journal (articles only); author's original; ; after publication|
|Publication indicators||= 1; : 2018 = 0.65; : 2018 = 2.527 (2) - 2018=1.873 (5)|
|Citation count*||2 (2020-06-05)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.