Possible sensor applications of selected DNA–surfactant complexes

Ewelina Nowak , Anna Wisła-Świder , Gohar Khachatryan , Maciej Fiedorowicz , Krzysztof Danel

Abstract

Although much research has been performed on DNA complexes carrying long alkyl chains (C10, C16, and C18), there is no information about physicochemical characterization of synthesized composites with allyl imidazole-based ionic liquids and quaternary ammonium salts with n-butyl chains. Here, complexes were synthesized by ion-exchange reactions between sonicated DNA and three ionic liquids (ILs) formed from two imidazole-based compounds, 1-allyl-3-methylimidazolium bromide (Amim) or 1-butyl-3-methylimidazolium bromide (Bmim), and from the quaternary ammonium salt tetra-n-butylammonium bromide (TBAB). Signals in UV–Vis, IR, and CD spectra indicating inclusion of small molecules into the DNA structure confirmed the formation of DNA complexes. Both IR and CD spectra indicated that the B-form conformation of the DNA did not change after the formation of the complexes. Similarly, X-ray diffraction patterns revealed that the formation of IL–DNA complexes did not change the structure of native B-form DNA. Molecular weight (Mw) and radii of gyration (Rg) values of IL–DNA complex chains, established by high-performance size exclusion chromatography coupled with multiangle-laser light-scattering with a differential refractive index detector, were significantly lower than those values found for native DNA molecules due to DNA fragmentation by sonication during complex formation and the direct effects of the IL on the DNA. Scanning electron microscopy images indicate the formation of nanofibres in DNA-Amim and DNA-Bmim complexes, whereas the formation of nanowires was found in samples of DNA-TBAB complexes. Changes in optical properties confirmed by UV and photoluminescence make DNA–IL complexes potential candidates for biosensor application.
Author Ewelina Nowak (FoFT / IoCh)
Ewelina Nowak,,
- Institute of chemistry
, Anna Wisła-Świder (FoFT / IoCh)
Anna Wisła-Świder,,
- Institute of chemistry
, Gohar Khachatryan (FoFT / IoCh)
Gohar Khachatryan ,,
- Institute of chemistry
, Maciej Fiedorowicz (FoFT / IoCh)
Maciej Fiedorowicz,,
- Institute of chemistry
, Krzysztof Danel (FoFT / IoCh)
Krzysztof Danel,,
- Institute of chemistry
Journal seriesEuropean Biophysics Journal With Biophysics Letters, [European Biophysics Journal], ISSN 0175-7571, e-ISSN 1432-1017, (N/A 70 pkt)
Issue year2019
Vol48
No4
Pages371-381
Publication size in sheets0.5
Keywords in EnglishDeoxyribonucleic acids, Biopolymers, Imidazoles, Ionic liquids, Ion-exchange reactions
ASJC Classification2700 General Medicine; 1304 Biophysics
DOIDOI:10.1007/s00249-019-01367-2
URL https://link.springer.com/content/pdf/10.1007%2Fs00249-019-01367-2.pdf
Internal identifierWTŻ/2019/40
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
Score (nominal)70
Score sourcejournalList
Publication indicators WoS Citations = 1; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.65; WoS Impact Factor: 2018 = 2.527 (2) - 2018=1.873 (5)
Citation count*2 (2020-06-05)
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back
Confirmation
Are you sure?