Estimation of uncertainty and validation of analytical procedures as a quality control tool the evaluation of uncertainty for amino acid analysis with ion-exchange chromatography - case study

Barbara Mickowska , Anna Sadowska-Rociek , Ewa Cieślik


The aim of this study was to assess the importance of validation and uncertainty estimation related to the results of amino acid analysis using the ion-exchange chromatography with post-column derivatization technique. The method was validated and the components of standard uncertainty were identified and quantified to recognize the major contributions to uncertainty of analysis. Estimated relative extended uncertainty (k=2, P=95%) varied in range from 9.03% to 12.68%. Quantification of the uncertainty components indicates that the contribution of the calibration concentration uncertainty is the largest and it plays the most important role in the overall uncertainty in amino acid analysis. It is followed by uncertainty of area of chromatographic peaks and weighing procedure of samples. The uncertainty of sample volume and calibration peak area may be negligible. The comparison of CV% with estimated relative uncertainty indicates that interpretation of research results can be misled without uncertainty estimation.
Author Barbara Mickowska (FoFT / DoToCaC)
Barbara Mickowska,,
- Department of Technology of Catering and Consumption
, Anna Sadowska-Rociek (FoFT / DoToCaC)
Anna Sadowska-Rociek,,
- Department of Technology of Catering and Consumption
, Ewa Cieślik (FoFT / DoToCaC)
Ewa Cieślik,,
- Department of Technology of Catering and Consumption
Journal seriesThe Journal of Microbiology, Biotechnology and Food Sciences, ISSN 1338-5178, (B 7 pkt)
Issue year2013
NoSpecial issue 1
Publication size in sheets0.5
Keywords in Englishamino acid analysis, combined standard uncertainty, expanded uncertainty
ASJC Classification1312 Molecular Biology; 2404 Microbiology; 1106 Food Science; 1305 Biotechnology
Internal identifierWTŻ/2013/108
Languageen angielski
Not used for evaluationyes
Score (nominal)0
Score sourcejournalList
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