Genetic Variability in the Loci of FABP4, PPARγ and SCD Genes of Sheep Breeds Raised for Different Purposes

Mirosław Kucharski , Urszula Kaczor , Katarzyna Piórkowska


The present study was undertaken to analyse the genetic variation in coding sequences, splicing sites and regulatory sequences of FABP4, PPARγ and SCD genes in five breeds of sheep raised in Poland with different purposes: meat (Suffolk, Ile de France), dairy/prolific (Olkuska sheep, Kołuda) and primitive breeds (Polish Mountain Sheep). To identify genetic variability HRM-PCR, sequencing and PCR-RFLP method were used. Determining the genetic structure of the mentioned genes revealed six SNPs: FABP4 – c.73 + 13A>T and c.348 + 85G>A, PPARγ – c.391 – 66C>T (c.481 – 66C>T) and c.529 + 27G>C (c.619 + 27G>C), SCD – c.*945G>A and c.*1116A>G. For the c.*1116A>G SNP, a potential association with specific sheep body type and breeding purpose was found. In turn, the substitution c.*945G>A located in the regulatory region of the 3'-UTR of the Ovis aries SCD gene was identified for the first time. Based on the relationship demonstrated between sheep body constitution and productive type (dairy, meat) and the polymorphism of the SCD gene, further research is needed. The correlation between c.*1116A>G polymorphism and growth rate, slaughter and carcass value as well as meat quality of lambs needs to be studied in more detail. Such studies may lead to more effective selection processes in sheep breeding in the future.
Author Mirosław Kucharski (FoAS / DoAB)
Mirosław Kucharski,,
- Department of Animal Biotechnology
, Urszula Kaczor (FoAS / DoAB)
Urszula Kaczor,,
- Department of Animal Biotechnology
, Katarzyna Piórkowska
Katarzyna Piórkowska,,
Journal seriesAnnals of Animal Science, ISSN 1642-3402, e-ISSN 2300-8733, (N/A 100 pkt)
Issue year2019
Publication size in sheets0.85
Keywords in EnglishFABP4, PPARγ, SCD, polymorphism, sheep
ASJC Classification3403 Food Animals; 3404 Small Animals; 1103 Animal Science and Zoology
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa - Użycie Niekomercyjne - Bez utworów zależnych (CC-BY-NC-ND); after publication
Genetic Variability in the Loci of FABP4, PPARγ and SCD Genes of Sheep Breeds Raised for Different Purposes of 12-11-2019
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Score (nominal)100
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.869; WoS Impact Factor: 2018 = 1.515 (2) - 2018=1.246 (5)
Citation count*
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FinansowanieResearch funded by: DS-3242/KBZ/17.
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