Ophiostomatoid fungi associated with hardwood-infesting bark and ambrosia beetles in Poland: Taxonomic diversity and vector specificity

Robert Jankowiak , Beata Strzałka , Piotr Bilański , Magdalena Kacprzyk , Piotr Wieczorek , Riikka Linnakoski

Abstract

Fungi in the orders Ophiostomatales and Microascales (Ascomycota), often designated as ophiostomatoid fungi, are frequent associates of scolytine bark and ambrosia beetles that colonize hardwood and coniferous trees. Several species, e.g., Ophiostoma novo-ulmi, are economically damaging pathogens of trees. Because little is known regarding the ophiostomatoid fungi in Europe, we have explored the diversity of these fungi associated with hardwood-infesting beetles in Poland. This study aims to clarify the associations between fungi in the genera Ambrosiella, Graphium (Microascales), Graphilbum, Leptographium, Ophiostoma and Sporothrix (Ophiostomatales) and their beetle vectors in hardwood ecosystems. Samples associated with 18 bark and ambrosia beetle species were collected from 11 stands in Poland. Fungi were isolated from adult beetles and galleries. Isolates were identified based on morphology, DNA sequence comparisons for five gene regions (ITS, LSU, ßT, TEF 1-α, and CAL) and phylogenetic analyses. In total, 36 distinct taxa were identified, including 24 known and 12 currently unknown species. Several associations between fungi and bark and ambrosia beetles were recorded for the first time. In addition, associations between Dryocoetes alni, D. villosus, Hylesinus crenatus, Ernoporus tiliae, Pteleobius vittatus and ophiostomatoid fungi were reported for the first time, and Sporothrix eucastanea was reported for the first time outside of the USA. Among the species of Ophiostomatales, 14 species were in Ophiostoma s. l., two species were in Graphilbum, nine species were in Sporothrix, and seven species were in Leptographium s. l. Among the species of Microascales, three species were in Graphium, and one was in Ambrosiella. Twenty taxa were present on the beetles and in the galleries, twelve only on beetles, and four only in galleries. Bark and ambrosia beetles from hardwoods appear to be regular vectors, with ophiostomatoid fungi present in all the beetle species. Most ophiostomatoid species had a distinct level of vector/host specificity, although Ophiostoma quercus, the most frequently encountered species, also had the greatest range of beetle vectors and tree hosts. Plant pathogenic O. novo-ulmi was found mainly in association with elm-infesting bark beetles (Scolytus multistriatus, S. scolytus, and P. vittatus) and occasionally with H. crenatus on Fraxinus excelsior and with Scolytus intricatus on Quercus robur.
Author Robert Jankowiak (FoF / FEPI / DoFFMaPoT)
Robert Jankowiak,,
- DEPARTMENT OF FOREST FITOPATOLOGY, MYKOLOGY AND PHYSIOLOGY OF TREES
, Beata Strzałka (FoF / FEPI)
Beata Strzałka,,
- Institute of Forest Ecosystem Protection
, Piotr Bilański (FoF / FEPI / DoFPEaC)
Piotr Bilański,,
- DEPARTMENT OF FOREST PROTECTION, ENTOMOLOGY AND FOREST CLIMATOLOGY
, Magdalena Kacprzyk (FoF / FEPI / DoFPEaC)
Magdalena Kacprzyk,,
- DEPARTMENT OF FOREST PROTECTION, ENTOMOLOGY AND FOREST CLIMATOLOGY
, Piotr Wieczorek (FoF / FEPI)
Piotr Wieczorek,,
- Institute of Forest Ecosystem Protection
, Riikka Linnakoski
Riikka Linnakoski,,
-
Journal seriesFungal Ecology, ISSN 1754-5048, e-ISSN 1878-0083, (N/A 100 pkt)
Issue year2019
Vol39
Pages152-167
Publication size in sheets0.75
Keywords in EnglishBark beetles, Ambrosia beetles, Hardwoods, Microascales, Ophiostomatales
ASJC Classification1105 Ecology, Evolution, Behavior and Systematics; 1110 Plant Science; 2302 Ecological Modelling; 2303 Ecology
DOIDOI:10.1016/j.funeco.2019.02.001
URL https://www.sciencedirect.com/science/article/pii/S1754504818301995
Languageen angielski
Score (nominal)100
Score sourcejournalList
Publication indicators WoS Citations = 2; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.247; WoS Impact Factor: 2018 = 3.99 (2) - 2018=3.845 (5)
Citation count*
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FinansowanieThe study was supported financially by the National Science Centre, Poland (contract No. UMO-2014/15/B/NZ9/00560).
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