The use of human and bovine commercial media for oocyte maturation and embryo development in the domestic cat (Felis catus)

Sylwia Prochowska , Wojciech Niżański , Agnieszka Partyka , Joanna Kochan , Wiesława Młodawska , Agnieszka Nowak , Józef Skotnicki , Teresa Grega , Marcin Pałys


The aim of this study was to examine the suitability of commercial media designed for humans and cattle for oocyte maturation and embryo culture in the domestic cat. In Exp. I, feline oocytes collected ex vivo were subjected to in vitro maturation in a laboratory‐made culture medium (based on M199) or a commercial medium designed for cattle cells (BO‐IVM®). In Exp. II, ICSI‐derived feline embryos were cultured for 7 days in a commercial human (Continuous Single Culture®) or bovine (BO‐EC®) cell medium. The rates of cleavage, morula and blastocyst formation were evaluated at 24 hr, 6 days and 7 days after ICSI, respectively, and compared between experimental groups. At the end of culture, embryos were assessed for viability and apoptotic changes. In Exp. I, no statistically significant difference in oocyte maturation outcome between laboratory‐made (52.7%) and commercial media (58.9%) was observed. However, the use of a commercial medium prepared for use with bovine cells resulted in a significantly lower variance of the maturation rate. In Exp. II, no statistically significant differences between two commercial media were observed for cleavage (67.5% and 64.5%), morula (39.3% and 47.1%) and blastocyst rates (25.0% and 19.6%), as well as for the percentage of late apoptotic blastomeres. Morulae cultured in medium marketed for humans exhibited significantly more early apoptotic (43.2 ± 31.2% vs. 23.4 ± 23.2%) and necrotic (60.6 ± 47.6% vs. 29.4 ± 22.6%) blastomeres. In conclusion, both commercial media tested are suitable for in vitro oocyte maturation and embryo culture procedures in cats. It is remarkable that a culture medium designed for use in cattle for in vitro maturation of cat oocytes provides more reproducible results.
Author Sylwia Prochowska
Sylwia Prochowska,,
, Wojciech Niżański
Wojciech Niżański,,
, Agnieszka Partyka
Agnieszka Partyka,,
, Joanna Kochan (FoAS / DoARaA)
Joanna Kochan,,
- Department of Animal Reproduction and Anatomy
, Wiesława Młodawska (DoAS / HBZ-KRiAZ)
Wiesława Młodawska,,
- Department of Animal Reproduction and Anatomy
, Agnieszka Nowak (FoAS / IoVS)
Agnieszka Nowak,,
- Institute of Veterinary Sciences
, Józef Skotnicki
Józef Skotnicki,,
, Teresa Grega
Teresa Grega,,
, Marcin Pałys
Marcin Pałys,,
Journal seriesReproduction in Domestic Animals, ISSN 0936-6768, e-ISSN 1439-0531, (N/A 100 pkt)
Issue year2019
Publication size in sheets0.5
Keywords in Englishcat, commercial media, embryo culture, oocyte maturation
ASJC Classification1103 Animal Science and Zoology; 1305 Biotechnology; 1310 Endocrinology
Internal identifierWHiBZ/2019/25
Languageen angielski
Score (nominal)100
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 0.934; WoS Impact Factor: 2018 = 1.638 (2) - 2018=1.677 (5)
Citation count*
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FinansowanieThis study was performed with support of the Project funded by the National Center of Research and Development PBS3/B8/16/2015 ID 247575.
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