The use of human and bovine commercial media for oocyte maturation and embryo development in the domestic cat (Felis catus)
Sylwia Prochowska , Wojciech Niżański , Agnieszka Partyka , Joanna Kochan , Wiesława Młodawska , Agnieszka Nowak , Józef Skotnicki , Teresa Grega , Marcin Pałys
AbstractThe aim of this study was to examine the suitability of commercial media designed for humans and cattle for oocyte maturation and embryo culture in the domestic cat. In Exp. I, feline oocytes collected ex vivo were subjected to in vitro maturation in a laboratory‐made culture medium (based on M199) or a commercial medium designed for cattle cells (BO‐IVM®). In Exp. II, ICSI‐derived feline embryos were cultured for 7 days in a commercial human (Continuous Single Culture®) or bovine (BO‐EC®) cell medium. The rates of cleavage, morula and blastocyst formation were evaluated at 24 hr, 6 days and 7 days after ICSI, respectively, and compared between experimental groups. At the end of culture, embryos were assessed for viability and apoptotic changes. In Exp. I, no statistically significant difference in oocyte maturation outcome between laboratory‐made (52.7%) and commercial media (58.9%) was observed. However, the use of a commercial medium prepared for use with bovine cells resulted in a significantly lower variance of the maturation rate. In Exp. II, no statistically significant differences between two commercial media were observed for cleavage (67.5% and 64.5%), morula (39.3% and 47.1%) and blastocyst rates (25.0% and 19.6%), as well as for the percentage of late apoptotic blastomeres. Morulae cultured in medium marketed for humans exhibited significantly more early apoptotic (43.2 ± 31.2% vs. 23.4 ± 23.2%) and necrotic (60.6 ± 47.6% vs. 29.4 ± 22.6%) blastomeres. In conclusion, both commercial media tested are suitable for in vitro oocyte maturation and embryo culture procedures in cats. It is remarkable that a culture medium designed for use in cattle for in vitro maturation of cat oocytes provides more reproducible results.
|Journal series||Reproduction in Domestic Animals, ISSN 0936-6768, e-ISSN 1439-0531, (N/A 100 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||cat, commercial media, embryo culture, oocyte maturation|
|ASJC Classification||; ;|
|Publication indicators||= 0; : 2017 = 0.934; : 2018 = 1.638 (2) - 2018=1.677 (5)|
|Finansowanie||This study was performed with support of the Project funded by the National Center of Research and Development PBS3/B8/16/2015 ID 247575.|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.