Genetic structure and domestication of carrot (Daucus carota subsp. sativus) (Apiaceae)

Massimo Iorizzo , Douglas Senalik , Shelby Ellison , Dariusz Grzebelus , Pablo Cavagnaro , Charlotte Allender , Johanne Brunet , David Spooner , Allen van Deynze , Philipp Simon

Abstract

Premise of the study: Analyses of genetic structure and phylogenetic relationships illuminate the origin and domestication of modern crops. Despite being an important worldwide vegetable, the genetic structure and domestication of carrot (Daucus carota) is poorly understood. We provide the first such study using a large data set of molecular markers and accessions that are widely dispersed around the world. Methods: Sequencing data from the carrot transcriptome were used to develop 4000 single nucleotide polymorphisms (SNPs). Eighty-four genotypes, including a geographically well-distributed subset of wild and cultivated carrots, were genotyped using the KASPar assay. Key results: Analysis of allelic diversity of SNP data revealed no reduction of genetic diversity in cultivated vs. wild accessions. Structure and phylogenetic analysis indicated a clear separation between wild and cultivated accessions as well as between eastern and western cultivated carrot. Among the wild carrots, those from Central Asia were genetically most similar to cultivated accessions. Furthermore, we found that wild carrots from North America were most closely related to European wild accessions. Conclusions: Comparing the genetic diversity of wild and cultivated accessions suggested the absence of a genetic bottleneck during carrot domestication. In conjunction with historical documents, our results suggest an origin of domesticated carrot in Central Asia. Wild carrots from North America were likely introduced as weeds with European colonization. These results provide answers to long-debated questions of carrot evolution and domestication and inform germplasm curators and breeders on genetic substructure of carrot genetic resources.
Author Massimo Iorizzo
Massimo Iorizzo,,
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, Douglas Senalik
Douglas Senalik,,
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, Shelby Ellison
Shelby Ellison,,
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, Dariusz Grzebelus (FoH / DoGPBaSS)
Dariusz Grzebelus,,
- Department of Genetics, Plant Breeding and Seed Science
, Pablo Cavagnaro
Pablo Cavagnaro,,
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, Charlotte Allender
Charlotte Allender,,
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, Johanne Brunet
Johanne Brunet,,
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, David Spooner
David Spooner,,
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, Allen van Deynze
Allen van Deynze,,
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, Philipp Simon
Philipp Simon,,
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Journal seriesAmerican Journal of Botany, ISSN 0002-9122, (A 35 pkt)
Issue year2013
Vol100
No5
Pages930-938
Publication size in sheets0.5
Keywords in EnglishApiaceae, Daucus carota, domestication, genetic diversity, single nucleotide polymorphism (SNP)
ASJC Classification1110 Plant Science; 1311 Genetics; 1105 Ecology, Evolution, Behavior and Systematics
DOIDOI:10.3732/ajb.1300055
URL http://www.amjbot.org/content/100/5/930.full.pdf
Internal identifierWBIO/2013/108
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa - Użycie Niekomercyjne - Na tych samych warunkach (CC-BY-NC-SA); after publication
Score (nominal)35
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2014 = 1.271; WoS Impact Factor: 2013 = 2.463 (2) - 2013=2.843 (5)
Citation count*30 (2016-06-29)
Additional fields
FinansowanieD.G. was supported by the Polish National Science Center, project no. N N302 062236 from 2009–2011.
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