Endogenous progesterone and its cellular binding sites in wheat exposed to drought stress

Anna Janeczko , Jana Oklešťková , Agata Siwek , Michał Dziurka , Ewa Pociecha , Maciej Kocurek , Novak Ondrej

Abstract

Progesterone is a basic hormone that regulates the metabolism in mammals. The presence of this compound has also been found in certain plants. Itis believed that progesterone can regulate growth processes and resistance to stress, however, its precise role in plants remains unknown. The research conducted in this study was aimed at analyzing the content of endogenous progesterone and its cellular binding sites in the leaves of spring wheat exposed to drought. Changes were studied in two cultivars of wheat– a cultivar sensitive to drought (Katoda) and tolerant cultivar (Monsun). Plants had undergone periodic droughts during the seedling stage or in the phase of heading. The occurrence of free progesterone as well as its conjugated forms was observed in wheat studied. The amount of progesterone ranged from 0.2 to 5.8 pmol g FW−1 and was dependent on the cultivar, age of the plants, stage of development and fluctuated as a result of the exposure to drought. Cv. Katoda responded to a water deficit by lowering the amount of progesterone and cv. Monsun by increasing its level. Progesterone in plants grown in limited water conditions occurred primarily in a free form. While in the optimal watering conditions, some of its pool was found in the form of conjugates. In the spring wheat the occurrence of binding sites for progesterone was detected in cell membranes, cytoplasm and nuclei in the range of 10–36 fmol/mg of protein. The wheat cultivars tested, Monsun and Katoda, differ in their concentration of cellular binding sites for progesterone. This number varied in the individual fractions during different stages of plant development and due to the effect of drought stress. The number of binding sites for progesterone located in the membrane fraction of seedlings and flag leaves increased significantly under drought in the cv. Katoda (35–46%), but did not change in the cv. Monsun.Whereas the number of cytoplasmic progesterone binding sites increased during the drought in the cv. Monsun (about 50%), they did not change in the cv. Katoda. Changes in the amount of progesterone and its binding sites in the cell under the influence of drought were then different depending on whether the cultivar was tolerant or sensitive to drought. The possibility of utilizing these changes as markers of drought resistance is discussed. The results obtained suggest that progesterone is a part of wheat response to stress factors (drought).
Author Anna Janeczko
Anna Janeczko,,
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, Jana Oklešťková
Jana Oklešťková,,
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, Agata Siwek
Agata Siwek,,
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, Michał Dziurka
Michał Dziurka,,
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, Ewa Pociecha (FoAE / DoPP)
Ewa Pociecha,,
- Department of Plant Physiology
, Maciej Kocurek
Maciej Kocurek,,
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, Novak Ondrej
Novak Ondrej,,
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Journal seriesJournal of Steroid Biochemistry and Molecular Biology, ISSN 0960-0760, (A 30 pkt)
Issue year2013
No138
Pages384-394
Publication size in sheets0.5
Keywords in EnglishPlants Wheat, Progesterone,Putative receptors, Drought stress
ASJC Classification1307 Cell Biology; 1308 Clinical Biochemistry; 1310 Endocrinology; 1312 Molecular Biology; 1313 Molecular Medicine; 1303 Biochemistry; 2712 Endocrinology, Diabetes and Metabolism
DOIDOI:10.1016/j.jsbmb.2013.07.014
URL http://www.sciencedirect.com/science/article/pii/S096007601300143X/pdfft?md5=c8bd8356c1f2b75b7eb29f44b91901ed&pid=1-s2.0-S096007601300143X-main.pdf
Internal identifierWRE/2013/146
Languageen angielski
Score (nominal)30
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2013 = 1.226; WoS Impact Factor: 2013 = 4.049 (2) - 2013=3.282 (5)
Citation count*9 (2016-08-08)
Additional fields
FinansowanieCzechy-Grant No.LK21306; Polska Projekt 818/N-COST/2010/0(COST action FA 0605)
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