Micropropagation of Rubus and Ribes spp

Ewa Dziedzic , Joanna Jagła

Abstract

Micropropagation is the most appropriate method for large-scale production of Rubus and Ribes spp. The proliferation rate of Rubus spp. differs in shoot tips and nodal segments. The culture media used for raspberry and blackberry propagation are MS-based supplemented with different combination and ratio of plant growth regulators, depending on the stage of culture. The initiation medium containing 0.4 mg L(-1) BA and 0.1 mg L(-1) IBA is used to stabilize shoot cultures. In multiplication media, concentration of cytokinin is doubled. In vitro rooting of shoots is achieved on media supplemented with 1.0 mg L(-1) IBA. Ribes spp. cultures are initiated from shoot tips, meristem, or dormant buds on MS medium supplemented with 2.0 mg L(-1) BA, 0.5 mg L(-1) IBA, and 0.1 mg L(-1) GA(3.) After stabilization of shoot cultures in 3-4-week time, shoot multiplication is carried out on MS medium containing 1.0 mg L(-1) BA and 0.1 mg L(-1) IBA. Shoots 2 cm long are cultured to rooting on a medium amended with 2.0 mg L(-1) IBA and 5.0 mg L(-1) IAA. Rooted plantlets are transferred to universal peat substrate and acclimatized in the greenhouse.
Author Ewa Dziedzic (FoH / DoPaAp)
Ewa Dziedzic,,
- Department of Pomology and Apiculture
, Joanna Jagła
Joanna Jagła,,
-
Pages149-160
Publication size in sheets0.55
Book Lambardi Maurizio, Ozudogru Elif , Jain Shri (eds.): Protocols for micropropagation of selected economically-important horticultural plants, Methods in molecular biology, no. 994, 2013, Humana Press - Springer Science+Business Media, ISBN 978-1-62703-073-1, 490 p., DOI:10.1007/978-1-62703-074-8
Keywords in EnglishProliferation, Ribes, Rubus, Shoot culture, Small fruit
DOIDOI:10.1007/978-1-62703-074-8_11
URL http://www.springerprotocols.com/Full/doi/10.1007/978-1-62703-074-8_11?encCode=U0xQOjExXzgtNDcwLTMwNzI2LTEtODc5&q=$encoder.encode%28$!q%29&tokenString=DnrpEHv5I2clbeB/ulrE2Q==&access=denied
Internal identifierWBIO/2013/126
Languageen angielski
LicenseOther; author's original; Other open licence; after publication
Score (nominal)0
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