The Influence of Photoperiod on the Action of Exogenous Leptin on Gene Expression of Proinflammatory Cytokines and Their Receptors in the Thoracic Perivascular Adipose Tissue (PVAT) in Ewes

Agata Krawczyńska , Andrzej P. Herman , Hanna Antushevich , Joanna Bochenek , Karolina Wojtulewicz , Dorota Zięba-Przybylska

Abstract

Leptin resistance is either a condition induced by human obesity or a natural phenomenon associated with seasonality in ruminants. In the cardiovascular system, the leptin resistance state presence is a complex issue. Moreover, the perivascular adipose tissue (PVAT) appears to be crucial as a source of proinflammatory cytokines and as a site of interaction for leptin contributing to endothelium dysfunction and atherosclerosis progression. So the aim of this study was to examine the influence of the photoperiod on the action of exogenous leptin on gene expression of selected proinflammatory cytokines and their receptors in thoracic PVAT of ewe with or without prior lipopolysaccharide (LPS) stimulation. The experiment was conducted on 48 adult, female ewes divided into 4 group ( in each): control, with LPS intravenous (iv.) injection (400 ng/kg of BW), with leptin iv. injection (20 μg/kg BW), and with LPS and 30-minute-later leptin injection, during short-day (SD) and long-day (LD) seasons. Three hours after LPS/control treatment, animals were euthanized to collect the PVAT adherent to the aorta wall. The leptin injection enhanced IL1B gene expression only in the LD season; however, in both seasons leptin injection intensified LPS-induced increase in IL1B gene expression. IL1R2 gene expression was increased by leptin injection only in the SD season. Neither IL6 nor its receptor and signal transducer gene expressions were influenced by leptin administration. Leptin injection increased TNFA gene expression regardless of photoperiodic conditions. Only in the SD season did leptin treatment increase the gene expression of both TNFα receptors. To conclude, leptin may modulate the inflammatory reaction progress in PVAT. In ewe, the sensitivity of PVAT on leptin action is dependent upon the photoperiodic condition with stronger effects stated in the SD season.
Author Agata Krawczyńska
Agata Krawczyńska,,
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, Andrzej P. Herman
Andrzej P. Herman,,
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, Hanna Antushevich
Hanna Antushevich,,
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, Joanna Bochenek
Joanna Bochenek,,
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, Karolina Wojtulewicz
Karolina Wojtulewicz,,
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, Dorota Zięba-Przybylska (FoAS / DoAB)
Dorota Zięba-Przybylska,,
- Department of Animal Biotechnology
Journal seriesMediators of Inflammation, ISSN 0962-9351, e-ISSN 1466-1861, (N/A 100 pkt)
Issue year2019
VolVolume 2019
Pages1-12
Publication size in sheets0.55
Article number7129476
ASJC Classification1307 Cell Biology; 2403 Immunology
DOIDOI:10.1155/2019/7129476
URL http://downloads.hindawi.com/journals/mi/2019/7129476.pdf
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
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The Influence of Photoperiod on the Action of Exogenous Leptin on Gene Expression of Proinflammatory Cytokines and Their Receptors in the Thoracic Perivascular Adipose Tissue (PVAT) in Ewes of 15-11-2019
2,52 MB
Score (nominal)100
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.049; WoS Impact Factor: 2018 = 3.545 (2) - 2018=4.184 (5)
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FinansowanieThe research was supported by the Polish National Science Centre (Krakow, Poland) (Project SONATA No. 2013/11/D/NZ9/02536).
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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