Somaclonal variation in embryogenic cultures of silver fir (Abies alba Mill.)
AbstractThe somaclonal variation analysis was conducted on callus of 57 lines obtained by the method of somatic embryogenesis from six zygotic embryos (with different genotypes) of silver fir (Abies alba Mill.) located in two mountain regions in the south of Poland. The somaclonal variation at the DNA level was estimated using RAPD markers and the data produced were used to estimate the level of similarity using Jaccard’s coefficient. For RAPD analysis, 24 ten‐nucleotide primers from the groups OPA, OPB and OPG were used. Two genotypes deriving from Krynica and Myślenice showed high genetic similarity (Jaccard’s coefficient 0.74 and 0.83), which provides a substantial chance for producing firs with the parental genotype. The remaining four genotypes showed somaclonal variation (average Jaccard’s coefficient approx. 0.5). The significance in variation of the research sites was ascertained by the ANOVA statistical test, which showed the impact of genotype, type of medium and phytohormones included in it on the variation among the fir lines bred in vitro. The somaclonal variation data in silver fir could be useful for its propagation through in vitro culture, and in generating detailed genetic maps of this species.
|Journal series||Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology , ISSN 1126-3504, e-ISSN 1724-5575, (A 30 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Abies alba , RAPD, embryonal suspensor mass, somaclonal variation, somatic embryogenesis, tissue culture|
|Publication indicators||= 7|
|Citation count*||11 (2015-07-15)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.