Response of carrot protoplasts and protoplast-derived aggregates to selection using a fungal culture filtrate of Alternaria radicina

Ewa Grzebelus , Maria Kruk , Alicja Macko-Podgórni , Dariusz Grzebelus

Abstract

Protoplasts isolated from three accessions of cultivated carrot and 5-day-old protoplast-derived aggregates were subjected to selection to identify somaclonal variants with enhanced tolerance to the fungal disease black rot incited by Alternaria radicina. Different concentrations [1, 2, 3.5, 5, 10, 20, 35 and 50 % (v/v)] of a fungal culture filtrate (FCF) from 2-week-old liquid cultures of A. radicina were used. Protoplasts and aggregates were subjected to short-term selection for a period of 10 days. All FCF concentrations added to the cultures on the day of isolation decreased protoplast survival frequency and plating efficiency, while FCF applied 5 days later inhibited cell divisions in 5–50 % concentrations. The responses of protoplasts to the treatment were genotype dependent. Most R0 plants were regenerated in all accessions from cell lines grown with 1 % FCF, while only a few plants were produced from 2 to 3.5 % FCF-treated cultures of ‘Dolanka’ and the breeding line ‘9304B’, respectively. Nineteen-percent of putative stress-tolerant regenerants were tetraploids, while only 5 % tetraploids were observed in the control. The incidence of unique random amplified polymorphic DNA fragments indicating possible chromosomal rearrangements was low and did not differ among regenerants after selection and those derived from the control. Mobilization of miniature inverted repeat transposable elements was not observed. Some R0 individuals regenerated both from FCF-treated and untreated cultures showed lower susceptibility to A. radicina in a laboratory assay in comparison to control plants grown from seed. Regenerants from FCF-treated cultures showed lower frequency of flowering plants and a higher rate of male sterility. Pollen viability of the putative stress-tolerant regenerants varied over a wide range (6–98 %), independently of in vitro selection conditions. Our data suggest that A. radicina FCF may be feasible for the in vitro selection to generate plants with superior phenotypic performance against A. radicina.
Author Ewa Grzebelus (FoH / DoGPBaSS)
Ewa Grzebelus,,
- Department of Genetics, Plant Breeding and Seed Science
, Maria Kruk
Maria Kruk,,
-
, Alicja Macko-Podgórni (FoH / DoGPBaSS)
Alicja Macko-Podgórni,,
- Department of Genetics, Plant Breeding and Seed Science
, Dariusz Grzebelus (FoH / DoGPBaSS)
Dariusz Grzebelus,,
- Department of Genetics, Plant Breeding and Seed Science
Journal seriesPlant Cell Tissue and Organ Culture, ISSN 0167-6857, (A 35 pkt)
Issue year2013
Vol115
No2
Pages209-222
Publication size in sheets0.65
Keywords in EnglishBlack rot, Daucus carota, In vitro selection, Petiole assay, Plating efficiency, Pollen viability
ASJC Classification1108 Horticulture
DOIDOI:10.1007/s11240-013-0353-8
URL http://link.springer.com/content/pdf/10.1007%2Fs11240-013-0353-8.pdf
Internal identifierWBIO/2013/114
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa - Użycie Niekomercyjne (CC-BY-NC); after publication
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Response of carrot protoplasts and protoplast-derived aggregates to selection using a fungal culture filtrate of Alternaria radicina of 27-05-2019
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Score (nominal)35
Score sourcejournalList
Publication indicators WoS Citations = 4; Scopus SNIP (Source Normalised Impact per Paper): 2013 = 1.524; WoS Impact Factor: 2013 = 2.612 (2) - 2013=2.261 (5)
Citation count*1 (2016-06-29)
Additional fields
FinansowanieThis work was supported by the Polish Ministry of Science and Higher Education (grant no. N310 047 32/2394).
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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