Cadmium-induced oxidative stress in Prussian carp (Carassius gibelio Bloch) hepatopancreas: ameliorating effect of melatonin

Ewa Drąg-Kozak , Dorota Pawlica-Gosiewska , Katarzyna Gawlik , Magdalena Socha , Grzegorz Gosiewski , Ewa Łuszczek-Trojnar , Bogdan Solnica , Włodzimierz Popek

Abstract

The oxidative status of the hepatopancreas of Prussian carp females (Carassius gibelio) co-exposed to sublethal cadmium in water and melatonin was studied. The activities of antioxidant enzymes such as glutathione reductase (GR), glutathione peroxidase (GPx), and superoxide dismutase (SOD) as well as the concentration of reduced glutathione (GSH) were measured in homogenates of the hepatopancreas. Furthermore, concentrations of cadmium (Cd), zinc (Zn), copper (Cu), and iron (Fe) in the hepatopancreas were assayed. These females received melatonin implants and were exposed to 0.4 mg/L or 4.0 mg/L Cd in water for either a 13- or a 7-week period, followed by further 6 weeks of purification in clear water. Exposure to Cd influenced the increase in this metal concentration in fish hepatopancreas. In contrast, the fish exposed to cadmium with additional administration of melatonin had a lower accumulation of this metal. Exposure to Cd caused the increase in GSH content and the activity of GR, and a reduction in GPx activity, whereas the SOD activity varies depending on the exposure time on cadmium. In the hepatopancreas of fish treated with Cd alone, the content of Cu and Zn were increased and that of Fe was changed. After melatonin administration to Cd-exposed fish, a decrease in copper and zinc hepatopancreas content was noted. The present findings imply that melatonin co-treatment can effectively protect the fish against the toxic effects of cadmium on endogenous antioxidant status in hepatopancreas tissues and variations in metal concentration, such as Zn, Cu, and Fe.
Author Ewa Drąg-Kozak (DoAS / DoIaF)
Ewa Drąg-Kozak,,
- Department of Ichthyobiology and Fisheries
, Dorota Pawlica-Gosiewska
Dorota Pawlica-Gosiewska,,
-
, Katarzyna Gawlik
Katarzyna Gawlik,,
-
, Magdalena Socha (DoAS / DoIaF)
Magdalena Socha,,
- Department of Ichthyobiology and Fisheries
, Grzegorz Gosiewski (DoAS / DoIaF)
Grzegorz Gosiewski,,
- Department of Ichthyobiology and Fisheries
, Ewa Łuszczek-Trojnar (DoAS / DoIaF)
Ewa Łuszczek-Trojnar,,
- Department of Ichthyobiology and Fisheries
, Bogdan Solnica
Bogdan Solnica,,
-
, Włodzimierz Popek (FoAS / DoIaF)
Włodzimierz Popek,,
- Department of Ichthyobiology and Fisheries
Journal seriesEnvironmental Science and Pollution Research, ISSN 0944-1344, e-ISSN 1614-7499, (N/A 70 pkt)
Issue year2019
Vol26
No12
Pages12264-12279
Publication size in sheets0.75
Keywords in EnglishCadmium, Melatonin, Oxidative stress, Prussian carp, Bioaccumulation, Depuration
ASJC Classification2700 General Medicine; 2304 Environmental Chemistry; 2307 Health, Toxicology and Mutagenesis; 2310 Pollution
DOIDOI:10.1007/s11356-019-04595-3
URL https://link.springer.com/content/pdf/10.1007%2Fs11356-019-04595-3.pdf
Internal identifierWHiBZ/2019/16
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
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Cadmium-induced oxidative stress in Prussian carp (Carassius gibelio Bloch) hepatopancreas: ameliorating effect of melatonin of 26-04-2019
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Score (nominal)70
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.032; WoS Impact Factor: 2018 = 2.914 (2) - 2018=3.208 (5)
Citation count*
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FinansowanieThis work was supported by the Ministry of Science and Higher Education of the Republic of Poland project no. DS 3202/KIiR
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