Differential expression of cell-cell junction proteins in the testis, epididymis, and ductus deferens of domestic turkeys (Meleagris gallopavo) with white and yellow semen

L. Pardyak , A. Kamińska , M. Brzoskwinia , A. Hejmej , Małgorzata Kotula-Balak , J. Jankowski , A. Ciereszko , B. Bilińska

Abstract

Tight, adherens, and gap junctions are involved in the regulation of reproductive tissue function in male mammals. In birds, including domestic turkeys, intercellular interactions performed by junctional networks have not yet been studied. Furthermore, the cellular and molecular basis of yellow semen syndrome (YSS) in the turkey population remains poorly understood. Thus, the aim of the present study was 2-fold: first, to provide new information on the localization and expression of cell-cell junction proteins in the testis, epididymis, and ductus deferens of domestic turkeys and second, to compare expression of junctional protein genes between 2 turkey population, one that produces white normal semen (WNS) and the other that produces yellow abnormal semen. Expression of occludin, zonula occludens-1 (ZO-1), connexin 43 (Cx43), N- and E-cadherin, and β-catenin genes were investigated using 3 complementary techniques: quantitative real-time PCR, western blot, and immunohistochemistry. Compared to WNS testis, epididymis, and ductus deferens, YSS tissues exhibited downregulation of occludin and β-catenin mRNA (P < 0.05) and protein (P < 0.05 and P < 0.01, respectively) and upregulation of N- and E-cadherin mRNA (P < 0.001, P < 0.05, P < 0.01, respectively) and protein (P < 0.01, P < 0.05, and P < 0.05, respectively). In contrast, ZO-1 and Cx43 mRNA and protein were upregulated in YSS testis (P < 0.05 and P < 0.001, respectively) but not in epididymis and ductus deferens; both mRNAs and proteins were downregulated (P < 0.05) compared to the respective WNS epididymis and ductus deferens. Altered staining intensity of immunoreactive proteins in YSS vs. WNS reproductive tissue sections confirmed the gene expression results. The present study is the first to demonstrate altered levels of junctional protein gene expression in reproductive tissues of male YSS turkeys. These findings may suggest that subtle changes in junctional protein expression affect the microenvironment in which spermatozoa develop and mature and thus may have an impact on the appearance of yellow semen in domestic turkeys.
Author L. Pardyak
L. Pardyak,,
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, A. Kamińska
A. Kamińska,,
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, M. Brzoskwinia
M. Brzoskwinia,,
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, A. Hejmej
A. Hejmej,,
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, Małgorzata Kotula-Balak (VET)
Małgorzata Kotula-Balak,,
- University Centre for Veterinary Medicine UJ-UR
, J. Jankowski
J. Jankowski,,
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, A. Ciereszko
A. Ciereszko,,
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, B. Bilińska
B. Bilińska,,
-
Journal seriesPoultry Science, ISSN 0032-5791, e-ISSN 1525-3171, (N/A 140 pkt)
Issue year2020
Vol99
No1
Pages555-566
Publication size in sheets0.55
Keywords in Englishcell-cell junctional proteins, testis-epididymis-ductus deferens, domestic turkey, yellow semen syndrome
ASJC Classification2700 General Medicine; 1103 Animal Science and Zoology
DOIDOI:10.3382/ps/pez494
URL https://www.sciencedirect.com/science/article/pii/S0032579119579007/pdfft?md5=9cb18b07caa3a3f892783ded2a5332bf&pid=1-s2.0-S0032579119579007-main.pdf
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa - Użycie Niekomercyjne - Bez utworów zależnych (CC-BY-NC-ND); after publication
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Differential expression of cell-cell junction proteins in the testis, epididymis, and ductus deferens of domestic turkeys (Meleagris gallopavo) with white and yellow semen of 20-01-2020
8,67 MB
Score (nominal)140
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.38; WoS Impact Factor: 2018 = 2.027 (2) - 2018=2.537 (5)
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