Bulb organogenesis of Tulipa tarda in vitro cultures in relation to light environment

Małgorzata Maślanka , Barbara Prokopiuk

Abstract

Here organogenesis of tarda tulip (Tulipa tarda Stapf.) from callus explants is presented. The callus tissue was cultivated on MS media containing 3% or 6% sucrose and either no addition of BAP (6-benzyl-aminopurine) or supplemented with 0.5 μM BAP. The cultures were maintained under a 16 h photoperiod under white, red or blue fluorescent light, at 20 ± 2°C for 12 weeks. This study aimed to determine the most suitable light conditions and medium composition for seed-derived callus explants in order to obtain an efficient formation of adventitious bulbs. There were no differences between the spectra of light in differentiating adventitious bulbs. Explants cultured in darkness (control), on 0.5 µM BAP and 3% sucrose, formed the highest number of adventitious bulbs. The efficiency of adventitious organogenesis amounted to 36.6 bulbs per 1 g of callus tissue. The fresh weight of biomass, cultured in these conditions, increased within 12 weeks from 1 to 6.99 g. Supplementation with BAP of the medium containing 3% sucrose promoted the formation of bulbs, but in the case of the medium with 6% sucrose, BAP had an adverse influence under every type of light. The obtained results provide a useful protocol for the micropropagation of T. tarda, which can be used commercially for rapid and cost-effective production of the tulip.
Author Małgorzata Maślanka (FoBaH / DoDP)
Małgorzata Maślanka,,
- Department of Decorative Plants
, Barbara Prokopiuk (FoBaH / DoDP)
Barbara Prokopiuk,,
- Department of Decorative Plants
Journal seriesActa Agriculturae Scandinavica Section B-Soil and Plant Science, [Acta Agriculturae Scandinavica - Section B Soil and Plant Science], ISSN 0906-4710, e-ISSN 1651-1913, (N/A 40 pkt)
Issue year2019
Vol69
No5
Pages398-404
Publication size in sheets0.5
Keywords in EnglishTulip, micropropagation, bulbing, callus explants, organogenesis
ASJC Classification1102 Agronomy and Crop Science; 1111 Soil Science
DOIDOI:10.1080/09064710.2019.1583361
URL https://www.tandfonline.com/doi/full/10.1080/09064710.2019.1583361?scroll=top&needAccess=true
Languageen angielski
Score (nominal)40
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.619; WoS Impact Factor: 2018 = 0.81 (2) - 2018=0.947 (5)
Citation count*
Additional fields
FinansowanieThis study was financed by the Polish Ministry of Science and Higher Education [DS 3500/KRO/2016].
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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