The association of Boeremia lilacis with necrotic lesions on shoots and leaf petioles and its pathogenicity towards Fraxinus excelsior

Tadeusz Kowalski , Wojciech Kraj , Bartłomiej Bednarz , Robert Rossa

Abstract

This paper reports the results of the study on the new type of necrotic lesion observed on epicormic shoots and leaf petioles of Fraxinus excelsior showing ash decline symptoms in stands of the Miechów Forest District, southern Poland. The performed symptom analysis included: shape and size of necroses, discoloration of necrotic tissue and the occurrence of fungal fructification. The same species of fungus, which was initially identified as Boeremia exigua, was isolated from 97% of epicormic shoots and from 82% of leaf petioles. However, detailed morphological and physiological (NaOH test) analyses and combined ITS, ACT, TUB and EF1-α phylogeny proved these isolates to belong to Boeremia lilacis, the species known so far as Syringa vulgaris pathogen occurring occasionally also on Forsythia hybrids or Philadelphus. Fraxinus excelsior is reported for the first time as a host for this fungus. To clarify the possible role of B. lilacis in development of necrotic lesions on ash, 10 randomly selected isolates were tested for their pathogenicity toward young F. excelsior plants using the method of artificial wound inoculation under field conditions. The isolates used in the test caused tissue necroses on all 60 inoculated shoots and all 60 inoculated leaf petioles. Necrotic lesions developed as a result of artificial inoculation corresponded largely to the symptoms following natural infections
Author Tadeusz Kowalski (FoF / FEPI / DoFFMaPoT)
Tadeusz Kowalski,,
- DEPARTMENT OF FOREST FITOPATOLOGY, MYKOLOGY AND PHYSIOLOGY OF TREES
, Wojciech Kraj (FoF / FEPI / DoFFMaPoT)
Wojciech Kraj,,
- DEPARTMENT OF FOREST FITOPATOLOGY, MYKOLOGY AND PHYSIOLOGY OF TREES
, Bartłomiej Bednarz (FoF / FEPI / DoFPEaC)
Bartłomiej Bednarz,,
- DEPARTMENT OF FOREST PROTECTION, ENTOMOLOGY AND FOREST CLIMATOLOGY
, Robert Rossa (FoF / FEPI / DoFPEaC)
Robert Rossa,,
- DEPARTMENT OF FOREST PROTECTION, ENTOMOLOGY AND FOREST CLIMATOLOGY
Journal seriesEuropean Journal of Plant Pathology, ISSN 0929-1873, e-ISSN 1573-8469, (N/A 100 pkt)
Issue year2019
Vol154
No4
Pages961-974
Publication size in sheets0.65
Keywords in EnglishBoeremia lilacis, Pathogenicity assay, Necrotic lesion
Keywords in original languageBoeremia lilacis, Pathogenicity assay, Necrotic lesion
ASJC Classification1102 Agronomy and Crop Science; 1108 Horticulture; 1110 Plant Science
Abstract in original languageThis paper reports the results of the study on the new type of necrotic lesion observed on epicormic shoots and leaf petioles of Fraxinus excelsior showing ash decline symptoms in stands of the Miechów Forest District, southern Poland. The performed symptom analysis included: shape and size of necroses, discoloration of necrotic tissue and the occurrence of fungal fructification. The same species of fungus, which was initially identified as Boeremia exigua, was isolated from 97% of epicormic shoots and from 82% of leaf petioles. However, detailed morphological and physiological (NaOH test) analyses and combined ITS, ACT, TUB and EF1-α phylogeny proved these isolates to belong to Boeremia lilacis, the species known so far as Syringa vulgaris pathogen occurring occasionally also on Forsythia hybrids or Philadelphus. Fraxinus excelsior is reported for the first time as a host for this fungus. To clarify the possible role of B. lilacis in development of necrotic lesions on ash, 10 randomly selected isolates were tested for their pathogenicity toward young F. excelsior plants using the method of artificial wound inoculation under field conditions. The isolates used in the test caused tissue necroses on all 60 inoculated shoots and all 60 inoculated leaf petioles. Necrotic lesions developed as a result of artificial inoculation corresponded largely to the symptoms following natural infections
DOIDOI:10.1007/s10658-019-01715-0
URL https://link.springer.com/content/pdf/10.1007%2Fs10658-019-01715-0.pdf
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
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The association of Boeremia lilacis with necrotic lesions on shoots and leaf petioles and its pathogenicity towards Fraxinus excelsior of 23-09-2019
1,58 MB
Score (nominal)100
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.91; WoS Impact Factor: 2018 = 1.744 (2) - 2018=1.754 (5)
Citation count*
Additional fields
FinansowanieThis work was supported by two projects concerning ash decline: project nos. 2011/03/B/NZ9/00078 and 2016/21/B/NZ9/01226, financed by the National Science Centre, Poland.
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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