A new liquid chromatography method with charge aerosol detector (CAD) for the determination of phospholipid classes. Application to milk phospholipids

Grzegorz Kiełbowicz , Piotr Micek , Czesław Wawrzeńczyk

Abstract

A new rapid method for the quantitative analysis of five classes of phospholipids (PLs) (phosphatidylcholine—PC, lysophosphatidylcholine—LPC, phosphatidylethanolamine—PE and phosphatidylserine—PS and phosphatidylinositol—PI) using liquid chromatography with charge aerosol detector (CAD) is described. The separation of the compounds of interest was achieved on a diol stationary phase with a mobile phase consisting of 13% HCOOH, hexane and 2-propanol in 19 min elution program, including 10 min equilibration of the column. The method was applied to characterize the phospholipid fractions of cow milk. PLs present in cow milk were separated by solid-phase extraction (SPE) procedure with Si cartridges before LC analysis with recovery ranging from 95.3% to 104.4%. The use of CAD detection of the eluted compounds was precise, linear and sensitive.
Author Grzegorz Kiełbowicz
Grzegorz Kiełbowicz,,
-
, Piotr Micek (DoAS / DoANaFS)
Piotr Micek,,
- Department of Animal Nutrition and Feed Science
, Czesław Wawrzeńczyk
Czesław Wawrzeńczyk,,
-
Journal seriesTalanta, ISSN 0039-9140, (A 40 pkt)
Issue year2013
Vol105
Pages28-33
Publication size in sheets0.5
Keywords in EnglishMilk, Phospholipids, SPE, Liquid chromatography, Charge aerosol detector (CAD)
ASJC Classification1600 General Chemistry
DOIDOI:10.1016/j.talanta.2012.11.051
URL http://www.sciencedirect.com/science/article/pii/S0039914012010041
Internal identifierWHiBZ/2013/73
Languageen angielski
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A new liquid chromatography method with charge aerosol detector (CAD) for the determination of phospholipid classes. Application to milk phospholipids 276 KB
Score (nominal)40
Score sourcejournalList
Publication indicators WoS Citations = 19; Scopus SNIP (Source Normalised Impact per Paper): 2014 = 1.296; WoS Impact Factor: 2013 = 3.511 (2) - 2013=3.756 (5)
Citation count*8 (2016-01-29)
Additional fields
FinansowanieThis work was financed by European Union from the European Social Fund, Grant/III/55/2009
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