Wpływ komórek ziarnistych i pożywek hodowlanych na kompetencje mejotyczne oocytów bydła oraz ich zdolność do rozwoju po zapłodnieniu in vitro

Wiesława Młodawska , Ewa Płoszaj

Abstract

The aim of the study was to compare the effect of gonadotropic hormones and granulosa cells on the maturation and developmental capacity of cattle oocytes in vitro, as well as the effect of TCM 199 and DMEM/F12 media on the development of embryos obtained in co-culture with oviduct epithelial cells. Fertilization was performed with the use of frozen semen from 2 bulls. Twenty hours after insemination, presumptive zygotes were placed in co-culture with oviduct cells in a TCM 199 (TCM-KJ co-culture) or a DMEM/F12 medium (DMEM-KJ co-culture) and cultured for 7-9 days. Metaphase II was reached by 40% and 48% of oocytes cultured in the presence of granulosa cells and gonadotropins, respectively. Only embryos obtained from oocytes maturing in the presence of granulosa cells developed to the blastocyst stage. Considerably more dividing embryos were obtained when the presumptive zygotes were co-cultured with TCM-KJ (38.1%) rather than with DMEM-KJ (8.6%; P<0.01). This study showed that the presence of granulosa cells had no effect on the nuclear maturation of cattle oocytes, but increased their capacity for embryonic development. TCM 199 is much more useful than DMEM/F12 for the co-culture of cattle embryos with oviduct cells.
Author Wiesława Młodawska (DoAS / HBZ-KRiAZ)
Wiesława Młodawska,,
- Department of Animal Reproduction and Anatomy
, Ewa Płoszaj (DoAS / DoARaA)
Ewa Płoszaj,,
- Department of Animal Reproduction and Anatomy
Other language title versionsEffect of granulosa cells and culture media on the meiotic competence of cattle oocytes and their developmental capacity after in vitro fertilization
Journal seriesMedycyna Weterynaryjna, ISSN 0025-8628, (A 15 pkt)
Issue year2013
Vol69
No5
Pages291-293
Publication size in sheets0.3
Keywords in Englishcattle, oocytes, in vitro maturation, in vitro fertilization, co-culture
ASJC Classification3400 General Veterinary
URL http://medycynawet.edu.pl/images/stories/pdf/pdf2013/052013/201305291293.pdf
Internal identifierWHiBZ/2013/45
Languagepl polski
Score (nominal)15
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2014 = 0.141; WoS Impact Factor: 2013 = 0.196 (2) - 2015=0.162 (5)
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