Chlorophyll a Fluorescence Parameters of Hulled and Hull-less Barley (Hordeum vulgare L.) DH Lines Inoculated with Fusarium culmorum

Tomasz Warzecha , Edyta Skrzypek , Tadeusz Adamski , Maria Surma , Zygmunt Kaczmarek , Agnieszka Sutkowska

Abstract

Barley worldwide is affected seriously by Fusarium seedling blight (FSB) and Fusarium head blight (FHB) diseases caused by the Fusarium species. The objective of this study was to facilitate the resistance of hulled and hull-less barley at different growth stages to F. culmorum according to direct parameters: disease rating (DR), fresh weight of leaves and roots, kernel weight per spike, kernel number per spike, plump kernels, and indirect parameters - chlorophyll a fluorescence (CF). Plate assay, greenhouse and field tests were performed on 30 spring barley doubled haploid (DH) lines and their parents infected with Fusarium culmorum. Direct parameters proved that hulled genotypes show less symptoms. Most studied chlorophyll a fluorescence (CF) parameters (apart from DIo/CS – amount of energy dissipated from PSII for laboratory test, TRo/CS – amount of excitation energy trapped in PSII reaction centers, ETo/CS – amount of energy used for electron transport and RC/CS – number of active reaction centres in the state of fully reduced PSII reaction center in field experiment) were significantly affected by F. culmorum infection. In all experiments, hulled genotypes had higher values of CF parameters compared to hull-less ones. Significant correlations were detected between direct and indirect parameters and also between various environments. It was revealed that ABS/CS, TRo/CS, and RC/CS have significant positive correlation in greenhouse test and field experiment. Significant correlations suggest the possibility of applying the CF parameters in selection of barley DH lines resistant to F. culmorum infection.
Author Tomasz Warzecha (FoAE / DoPBaS)
Tomasz Warzecha,,
- Department of Plant Breeding and Seed Science
, Edyta Skrzypek
Edyta Skrzypek,,
-
, Tadeusz Adamski
Tadeusz Adamski,,
-
, Maria Surma
Maria Surma ,,
-
, Zygmunt Kaczmarek
Zygmunt Kaczmarek,,
-
, Agnieszka Sutkowska (FoAE / DoPBaS)
Agnieszka Sutkowska,,
- Department of Plant Breeding and Seed Science
Journal seriesPlant Pathology Journal, ISSN 1598-2254, e-ISSN 2093-9280, [1812-5387], (N/A 70 pkt)
Issue year2019
Vol35
No2
Pages112-124
Publication size in sheets0.6
Keywords in Englishchlorophyll a fluorescence, fungal infection, Fusarium head blight, Fusarium seedling blight, spring barley
DOIDOI:10.5423/PPJ.OA.07.2018.0124
URL http://www.ppjonline.org/journal/download_pdf.php?doi=10.5423/PPJ.OA.07.2018.0124
Internal identifierWRE/2019/51
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa - Użycie Niekomercyjne (CC-BY-NC); after publication
Score (nominal)70
Score sourcejournalList
Publication indicators WoS Citations = 0; WoS Impact Factor: 2018 = 1.294 (2) - 2018=1.705 (5)
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