Osteopontin and fatty acid binding protein in ifosfamide-treated rats

Łukasz Dobrek , Zbigniew Arent , Klaudia Nalik-Iwaniak , Kinga Fic , Marta Kopańska

Abstract

Introduction: Ifosfamide (IF) is a cytostatic that exhibits adverse nephrotoxic properties. Clinically, IF-in- duced nephrotoxicity takes various forms, depending on applied dose and length of treatment. Objectives: The aim of the study was to evaluate the two proteins: osteopontin (OP) and fatty acid binding protein (FABP), as markers of kidney function in rats treated with ifosfamide. Material and Methods: Rats receiving a single IF dose (250 mg/kg b.w.; group 1) or treated with five consecutive IF doses administrated on following days (50mg/kg b.w.; group 3), compared with control groups 2 and 4, respec- tively, were studied. Kidney function was assessed using classical (urea, creatinine) and novel (FABP, OP) labora- tory parameters and by histopathology. Results: Single IF dose administration resulted in signif- icant total proteinuria with urinary concentrations and 24-hour excretions of both FABP and OP comparable to the appropriate control. In rats treated with five consecutive IF doses, the urinary concentrations and 24-hour excretion of both FABP and OP were significantly higher compared to the appropriate control. The development of cystitis was revealed in groups 1 and 3, which was not accompanied by significant histopathological kidney damage. Conclusions: Both OP and FABP may be useful labora- tory markers of tubulopathy in the early stage of chronic nephrotoxicity of ifosfamide.
Author Łukasz Dobrek
Łukasz Dobrek,,
-
, Zbigniew Arent (VET / CfEaIM)
Zbigniew Arent,,
- Center for Experimental and Innovative Medicine
, Klaudia Nalik-Iwaniak (VET / CfEaIM)
Klaudia Nalik-Iwaniak,,
- Center for Experimental and Innovative Medicine
, Kinga Fic (VET / CfEaIM)
Kinga Fic,,
- Center for Experimental and Innovative Medicine
, Marta Kopańska
Marta Kopańska,,
-
Journal seriesOpen Medicine, [Open Medicine (Poland)], ISSN 2391-5463, (N/A 20 pkt)
Issue year2019
Vol14
No1
Pages561-571
Publication size in sheets0.5
Keywords in EnglishRats; Ifosfamide; Nephrotoxicity; Fatty acid binding protein; Osteopontin
ASJC Classification2700 General Medicine
DOIDOI:10.1515/med-2019-0063
URL https://www.degruyter.com/downloadpdf/j/med.2019.14.issue-1/med-2019-0063/med-2019-0063.xml
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa - Użycie Niekomercyjne - Bez utworów zależnych (CC-BY-NC-ND); after publication
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Osteopontin and fatty acid binding protein in ifosfamide-treated rats 1,78 MB
Score (nominal)20
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 0.264; WoS Impact Factor: 2018 = 1.221 (2) - 2018=0.952 (5)
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