Melatonin promotes seed germination under salinity and enhances the biosynthesis of steviol glycosides in Stevia rebaudiana Bertoni leaves

Magdalena Simlat , Agnieszka Szewczyk , Agata Ptak

Abstract

Melatonin (MEL) can act as a plant growth regulator and biostimulator in stressful situations. Using MEL in seed pretreatment also affects the future growth of plants. Therefore, this research investigated the effects of MEL on seed germination and seedling growth under NaCl in in vitro conditions. The additional effects of MEL on the accumulation of steviol glycosides (SGs) and on the expression of appropriate genes were also studied. Five μM of MEL was the best concentration for seed germination, while 20 μM exerted a positive impact on the biomass of stevia plantlets. NaCl significantly decreased seed germination, but MEL alleviated this effect when seeds were germinated in 50 mM of NaCl. Under salinity, the values of almost all morphological traits decreased as MEL concentration increased. The highest amounts of stevioside and rebaudioside A (Reb A) were observed as a result of treating seeds with 5 and 20 μM of MEL, respectively. When adding NaCl, positive impacts of MEL on the accumulation of both SGs were also observed. Expression analyses of the genes involved in SGs biosynthesis was explored in seeds and leaves, and the transcripts of key enzymes occurred in both the tissues. However, quantitative polymerase chain reaction (qPCR) analysis showed that all tested genes were upregulated in younger leaves, contrary to older ones. Also in younger, rather than older, leaves SG gene expression varied according to MEL concentration. This study, therefore, presents the promising potential of MEL for improving stevia seed germination under salinity conditions and for enhancing the production of SGs in stevia plants.
Author Magdalena Simlat (FoAaE / DoPoPBaSP)
Magdalena Simlat,,
- Department of Physiology of Plant Breeding and Seed Production
, Agnieszka Szewczyk - [Jagiellonian University (UJ)]
Agnieszka Szewczyk,,
-
- Uniwersytet Jagielloński w Krakowie
, Agata Ptak (FoAaE / DoPoPBaSP)
Agata Ptak,,
- Department of Physiology of Plant Breeding and Seed Production
Journal seriesPLoS ONE, ISSN 1932-6203, (N/A 100 pkt)
Issue year2020
No15(3)
Pages1-20
Publication size in sheets0.95
Article numbere0230755
ASJC Classification2700 General Medicine; 1100 General Agricultural and Biological Sciences; 1300 General Biochemistry, Genetics and Molecular Biology
DOIDOI:10.1371/journal.pone.0230755
URL https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0230755
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
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Melatonin promotes seed germination under salinity and enhances the biosynthesis of steviol glycosides in Stevia rebaudiana Bertoni leaves of 21-05-2020
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Score (nominal)100
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.111; WoS Impact Factor: 2018 = 2.776 (2) - 2018=3.337 (5)
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FinansowanieThe research was financed by the Ministry of Science and Higher Educationof the Republic of Poland. The funders had no role in study design, data collectionand analysis, decision to publish, or preparation of the manuscript.
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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