Expression of PC, PCK1, PCK2, LDHB, FBP1 and G6PC gens in the liver of cows in the transition from pregnancy to lactation

Paweł Górka , Beata Żelazowska , Krzysztof Słoniewski , Zygmunt M. Kowalski , Lech Zwierzchowski , Małgorzata Ostrowska


The effect of the transition of dairy cows from pregnancy to lactation on the expression of genes encoding enzymes involved in hepatic glucose metabolism was studied. Six Holstein-Friesian heifers were used for this study. Liver samples were collected by biopsy on day 7 before expected parturition (-7) and days 3 and 21 after parturition (+3 and +21, respectively). The mRNA levels of pyruvate carboxylase (PC), phosphoenolpyruvate carboxykinase 1 (PCK1), phosphoenolpyruvate carboxykinase 2 (PCK2), lactate dehydrogenase B (LDHB), fructose-1,6-bisphosphatase 1 (FBP1) and glucose-6-phosphatase (G6PC) were measured using quantitative real-time PCR. The expression of PC and PCK2 mRNA on day 3 of lactation was significantly higher than that on day 7 before parturition (P<0.05) and slightly higher than on day 21 postpartum. The LDHB gene showed the highest expression level on day 3 of lactation, as compared with day 7 prepartum (P<0.001) and day 21 postpartum (P<0.001). No differences were shown in PCK1, FBP1 and G6PC expression levels between pregnancy and early lactation.
Author Paweł Górka (DoAS / DoANaFS)
Paweł Górka,,
- Department of Animal Nutrition and Feed Science
, Beata Żelazowska
Beata Żelazowska,,
, Krzysztof Słoniewski
Krzysztof Słoniewski,,
, Zygmunt M. Kowalski (DoAS / DoANaFS)
Zygmunt M. Kowalski,,
- Department of Animal Nutrition and Feed Science
, Lech Zwierzchowski
Lech Zwierzchowski,,
, Małgorzata Ostrowska
Małgorzata Ostrowska,,
Journal seriesAnimal Science Papers and Reports, ISSN 0860-4037, (A 25 pkt)
Issue year2013
Publication size in sheets0.5
Keywords in Englishdairy cow, gene expression, glucose metabolism, liver
ASJC Classification3400 General Veterinary; 1311 Genetics; 1103 Animal Science and Zoology; 1305 Biotechnology
Internal identifierWHiBZ/2013/70
Languageen angielski
Additional file
Expression of PC, PCK1, PCK2, LDHB, FBP1 and G6PC gens in the liver of cows in the transition from pregnancy to lactation 329,87 KB
Score (nominal)25
Score sourcejournalList
Publication indicators WoS Citations = 3; Scopus SNIP (Source Normalised Impact per Paper): 2013 = 0.668; WoS Impact Factor: 2013 = 0.814 (2) - 2013=0.788 (5)
Citation count*
Additional fields
FinansowanieThis study was funded within Polish National Science Centre grants N N311 075 039 and N N311 632438
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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