Expression of PC, PCK1, PCK2, LDHB, FBP1 and G6PC gens in the liver of cows in the transition from pregnancy to lactation
Paweł Górka , Beata Żelazowska , Krzysztof Słoniewski , Zygmunt M. Kowalski , Lech Zwierzchowski , Małgorzata Ostrowska
AbstractThe effect of the transition of dairy cows from pregnancy to lactation on the expression of genes encoding enzymes involved in hepatic glucose metabolism was studied. Six Holstein-Friesian heifers were used for this study. Liver samples were collected by biopsy on day 7 before expected parturition (-7) and days 3 and 21 after parturition (+3 and +21, respectively). The mRNA levels of pyruvate carboxylase (PC), phosphoenolpyruvate carboxykinase 1 (PCK1), phosphoenolpyruvate carboxykinase 2 (PCK2), lactate dehydrogenase B (LDHB), fructose-1,6-bisphosphatase 1 (FBP1) and glucose-6-phosphatase (G6PC) were measured using quantitative real-time PCR. The expression of PC and PCK2 mRNA on day 3 of lactation was significantly higher than that on day 7 before parturition (P<0.05) and slightly higher than on day 21 postpartum. The LDHB gene showed the highest expression level on day 3 of lactation, as compared with day 7 prepartum (P<0.001) and day 21 postpartum (P<0.001). No differences were shown in PCK1, FBP1 and G6PC expression levels between pregnancy and early lactation.
|Journal series||Animal Science Papers and Reports, ISSN 0860-4037, (A 25 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||dairy cow, gene expression, glucose metabolism, liver|
|ASJC Classification||; ; ;|
|Publication indicators||= 3; : 2013 = 0.668; : 2013 = 0.814 (2) - 2013=0.788 (5)|
|Finansowanie||This study was funded within Polish National Science Centre grants N N311 075 039 and N N311 632438|
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