Fluorescent Banding Pattern and Species-Specific DNA Marker in Rumex thyrsiflorus Fingerh

Aleksandra Grabowska-Joachimiak , Dagmara Kwolek , Adam Kula , Paweł Marciniuk

Abstract

The object of this work was to analyze the karyotype structure of Rumex thyrsiflorus using differential fluorescent methods of chromosome staining (C-banding/DAPI and CMA3/DA/DAPI) and molecular sex markers. The results obtained were compared with data on the structure of the sex chromosomes and autosomes in R. acetosa, a model species in studies of sex determination and sex chromosome evolution in plants with an XX/XY1Y2 system. A high level of similarity was found in the sex chromosome structure of the 2 species, along with small differences in their autosomal complexes. It suggests that differentiation of these 2 closely related species was not accompanied by major structural changes within their sex chromosomes. Molecular tests, however, revealed differences in the composition of male-specific repetitive sequence RAYSII, occurring in the Y1 chromosome. Amplification of this sequence showed the presence of a single product (∼700 bp) in R. acetosa and of 2 products (∼600 bp and ∼700 bp) in R. thyrsiflorus. The longer product (∼700 bp) was also revealed in R. arifolius, another species closely related to R. acetosa. The shorter DNA fragment, characteristic of R. thyrsiflorus, differed from the common product by of a large indel with a length of 110 bp. This fragment may serve as a species-specific molecular marker useful in taxonomical and population studies as well as in further research on the sex chromosome differentiation in R. thyrsiflorus.
Author Aleksandra Grabowska-Joachimiak (FoAE / DoPBaS)
Aleksandra Grabowska-Joachimiak,,
- Department of Plant Breeding and Seed
, Dagmara Kwolek
Dagmara Kwolek,,
-
, Adam Kula (FoAE / DoPBaS)
Adam Kula,,
- Department of Plant Breeding and Seed
, Paweł Marciniuk
Paweł Marciniuk,,
-
Journal seriesCytogenetic and Genome Research, ISSN 1424-8581, (A 20 pkt)
Issue year2012
Vol137
No1
Pages70-77
Publication size in sheets0.5
Keywords in EnglishC-banding/DAPI-CMA3/DA/DAPI, male-specific sequence RAYSIL, molecular marker, Rumex acetosa, Rumex thyrsiflorus
DOIDOI:10.1159/000339383
URL https://www.researchgate.net/publication/227342606_Fluorescent_Banding_Pattern_and_Species-Specific_DNA_Marker_in_Rumex_thyrsiflorus_Fingerh
Internal identifierWBIO/2012/5
Languageen angielski
Score (nominal)20
Citation count*4 (2016-08-05)
Additional fields
FinansowanieThis work was supported by the grant N N303 598139 from the Polish Ministry of Science and Higher Education/National Science Center (NCN).
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