Prevalence of antibiotic-resistant Escherichia coli strains in selected rivers of Lesser Poland

Maria Jolanta Chmiel , Anna Lenart-Boroń

Abstract

This study aimed to determine the incidence of antibiotic-resistant strains of Escherichia coli in waters of three Lesser Poland rivers: Raba, Prądnik and Białka. The samples were collected from 30 sites—10 from each river along their course. Membrane filtration method was used to determine the water quality based on the prevalence of fecal coliforms in water. The contaminated samples were used to isolate Escherichia coli for further studies. After confirming the systematic position of randomly isolated strains, the antibiotic resistance tests were performed using the disk-diffusion method following the recommendations of the National Reference Centre for Susceptibility Testing. Escherichia coli were present in all rivers. Complete antibiograms were obtained for 83 isolates; 30 strains originating from the Prądnik river, 37 from Raba and 16 from Białka. A total of 67.47% strains were resistant to at least 1 tested antimicrobial (76.7% isolates from Prądnik, 73% from Raba and 37.5% from Białka), 33.7% of all strains were resistant to at least 5 antimicrobial agents, and one strain was resistant to even 13 antibiotics. The highest resistance was observed for ampicillin and the lowest for imipenem, fosfomycin and meropenem. The obtained results showed that the resistant strains of Escherichia coli are widely spread in the water environment, even in protected areas.
Author Maria Jolanta Chmiel (FoAE / DoM)
Maria Jolanta Chmiel,,
- Department of Microbiology
, Anna Lenart-Boroń (FoAaE / DoM)
Anna Lenart-Boroń,,
- Department of Microbiology
Journal seriesInternational Journal of Environmental Science and Technology, ISSN 1735-1472, e-ISSN 1735-2630, (N/A 70 pkt)
Issue year2019
Vol16
No1
Pages391-400
Publication size in sheets0.5
Keywords in EnglishAntibiotic resistance, Escherichia coli, Fecal coliforms, Surface water, Water quality
ASJC Classification1100 General Agricultural and Biological Sciences; 2304 Environmental Chemistry; 2305 Environmental Engineering
DOIDOI:10.1007/s13762-018-1681-3
URL https://link.springer.com/article/10.1007/s13762-018-1681-3
Internal identifierWRE/2018/27
Languageen angielski
Score (nominal)70
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.918; WoS Impact Factor: 2018 = 2.031 (2) - 2018=2.396 (5)
Citation count*
Additional fields
Udział procentowyM.J. Chmiel: 70%, A. Lenart-Boroń: 30%
FinansowanieThis study was funded by statutory measures of the University of Agriculture in Kraków, within a Grant No. DS 3102/KM/2013.
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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