Prevalence of antibiotic-resistant Escherichia coli strains in selected rivers of Lesser Poland
Maria Jolanta Chmiel , Anna Lenart-Boroń
AbstractThis study aimed to determine the incidence of antibiotic-resistant strains of Escherichia coli in waters of three Lesser Poland rivers: Raba, Prądnik and Białka. The samples were collected from 30 sites—10 from each river along their course. Membrane filtration method was used to determine the water quality based on the prevalence of fecal coliforms in water. The contaminated samples were used to isolate Escherichia coli for further studies. After confirming the systematic position of randomly isolated strains, the antibiotic resistance tests were performed using the disk-diffusion method following the recommendations of the National Reference Centre for Susceptibility Testing. Escherichia coli were present in all rivers. Complete antibiograms were obtained for 83 isolates; 30 strains originating from the Prądnik river, 37 from Raba and 16 from Białka. A total of 67.47% strains were resistant to at least 1 tested antimicrobial (76.7% isolates from Prądnik, 73% from Raba and 37.5% from Białka), 33.7% of all strains were resistant to at least 5 antimicrobial agents, and one strain was resistant to even 13 antibiotics. The highest resistance was observed for ampicillin and the lowest for imipenem, fosfomycin and meropenem. The obtained results showed that the resistant strains of Escherichia coli are widely spread in the water environment, even in protected areas.
|Journal series||International Journal of Environmental Science and Technology, ISSN 1735-1472, e-ISSN 1735-2630, (N/A 70 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Antibiotic resistance, Escherichia coli, Fecal coliforms, Surface water, Water quality|
|ASJC Classification||; ;|
|Publication indicators||= 0; : 2018 = 0.918; : 2018 = 2.031 (2) - 2018=2.396 (5)|
|Udział procentowy||M.J. Chmiel: 70%, A. Lenart-Boroń: 30%|
|Finansowanie||This study was funded by statutory measures of the University of Agriculture in Kraków, within a Grant No. DS 3102/KM/2013.|
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