The Change of Bacterial Spectrum after Storage of X. campestris pv. campestris Inoculated Cabbage Heads (Brassica oleracea var. capitata L.)
Lucia Ragasová , Eliška Peňázová , Filip Gazdík , Jakub Pečenka , Jana Čechová , Robert Pokluda , Miroslav Baránek , Dariusz Grzebelus , Aleš Eichmeier
AbstractChanges in the bacterial spectrum of cabbage heads after storage under commonly used storage conditions were examined in this study. Cabbage seeds (Brassica oleracea var. capitata L.) were artiﬁcially inoculated with X. campestris pv. campestris (Xcc), a serious pathogen of cruciferous plants causing black rot. Isolation of bacterial cultures from Xcc-inoculated and non-inoculated cabbage heads were carried out in two time points—at the day of harvest and after four months of storage. According to our previous research and literature reports, the most frequent genera of bacteriawerechosenforPCRtesting,i.e.,Bacilluscereusgroup,Bacillussubtilisgroup,Pseudomonassp., and X. campestris pv. campestris. A few of the obtained bacterial cultures were negative for the four above-mentioned species. In those, other bacteria were identiﬁed by 16S rRNA sequencing. In both Xcc-inoculated and non-inoculated cabbage heads, changes of the bacterial spectrum over time were observed. The severity of Xcc infection of heads increased after four months of storage. Bacillus speciesrepresentedthemostfrequentlyoccurringbacterialgenus. ThepresenceoftheBacillussubtilis group increased signiﬁcantly after storage in non-inoculated cabbage heads. The minor part of the other genera identiﬁed by sequencing in the ﬁrst sampling were not detected in the stored cabbage heads. This was associated with a possible antagonistic behavior of Pseudomonas sp. and Bacillus sp.
|Journal series||Agronomy, [Agronomy], ISSN 2073-4395, e-ISSN 2073-4395, (N/A 100 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||black rot; sequencing; post-harvest; storage; vegetable; Pseudomonas; cabbage; Bacillus|
|License||Journal (articles only); author's original; ; after publication|
|Publication indicators||: 2018 = 1.272; : 2018 = 2.259 (2)|
|Finansowanie||This work was supported from “Multidisciplinary research to increase application potential of nanomaterials in agricultural practice” (No. CZ.02.1.01/0.0/0.0/16_025/0007314). This research was also supported by the project LTC18009 provided by the Ministry of Education, Youth and Sports of the Czech Republic.|
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