The Change of Bacterial Spectrum after Storage of X. campestris pv. campestris Inoculated Cabbage Heads (Brassica oleracea var. capitata L.)

Lucia Ragasová , Eliška Peňázová , Filip Gazdík , Jakub Pečenka , Jana Čechová , Robert Pokluda , Miroslav Baránek , Dariusz Grzebelus , Aleš Eichmeier

Abstract

Changes in the bacterial spectrum of cabbage heads after storage under commonly used storage conditions were examined in this study. Cabbage seeds (Brassica oleracea var. capitata L.) were artificially inoculated with X. campestris pv. campestris (Xcc), a serious pathogen of cruciferous plants causing black rot. Isolation of bacterial cultures from Xcc-inoculated and non-inoculated cabbage heads were carried out in two time points—at the day of harvest and after four months of storage. According to our previous research and literature reports, the most frequent genera of bacteriawerechosenforPCRtesting,i.e.,Bacilluscereusgroup,Bacillussubtilisgroup,Pseudomonassp., and X. campestris pv. campestris. A few of the obtained bacterial cultures were negative for the four above-mentioned species. In those, other bacteria were identified by 16S rRNA sequencing. In both Xcc-inoculated and non-inoculated cabbage heads, changes of the bacterial spectrum over time were observed. The severity of Xcc infection of heads increased after four months of storage. Bacillus speciesrepresentedthemostfrequentlyoccurringbacterialgenus. ThepresenceoftheBacillussubtilis group increased significantly after storage in non-inoculated cabbage heads. The minor part of the other genera identified by sequencing in the first sampling were not detected in the stored cabbage heads. This was associated with a possible antagonistic behavior of Pseudomonas sp. and Bacillus sp.
Author Lucia Ragasová - University in Brno
Lucia Ragasová,,
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, Eliška Peňázová - University in Brno
Eliška Peňázová,,
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, Filip Gazdík - University in Brno
Filip Gazdík,,
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, Jakub Pečenka - University in Brno
Jakub Pečenka,,
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, Jana Čechová - University in Brno
Jana Čechová,,
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, Robert Pokluda - University in Brno
Robert Pokluda,,
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, Miroslav Baránek - University in Brno
Miroslav Baránek,,
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, Dariusz Grzebelus (FoBaH / Department of Plant Biology and Biotechnology) - University in Brno
Dariusz Grzebelus,,
- Department of Plant Biology and Biotechnology
, Aleš Eichmeier - University in Brno
Aleš Eichmeier,,
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Journal seriesAgronomy, [Agronomy], ISSN 2073-4395, e-ISSN 2073-4395, (N/A 100 pkt)
Issue year2020
Vol10
No3
Pages1-10
Publication size in sheets0.5
Article number443
Keywords in Englishblack rot; sequencing; post-harvest; storage; vegetable; Pseudomonas; cabbage; Bacillus
ASJC Classification1102 Agronomy and Crop Science
DOIDOI:10.3390/agronomy10030443
URL https://www.mdpi.com/2073-4395/10/3/443/pdf
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
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The Change of Bacterial Spectrum after Storage of X. campestris pv. campestris Inoculated Cabbage Heads (Brassica oleracea var. capitata L.) of 15-04-2020
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Score (nominal)100
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.272; WoS Impact Factor: 2018 = 2.259 (2)
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FinansowanieThis work was supported from “Multidisciplinary research to increase application potential of nanomaterials in agricultural practice” (No. CZ.02.1.01/0.0/0.0/16_025/0007314). This research was also supported by the project LTC18009 provided by the Ministry of Education, Youth and Sports of the Czech Republic.
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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